The effect of oxidative metabolism on spontaneous Pol zeta-dependent translesion synthesis in Saccharomyces cerevisiae.
Journal Article (Journal Article)
DNA lesions can stall or block high-fidelity polymerases, thus inhibiting replication. To bypass such lesions, low-fidelity translesion synthesis (TLS) polymerases can be used to insert a nucleotide across from the lesion or extend from a lesion:base mispair. When DNA repair is compromised in Saccharomyces cerevisiae, spontaneous DNA lesions can lead to a novel mutational event in which a frameshift is accompanied by one or more base pair substitutions. These "complex frameshifts" are dependent upon the TLS polymerase Pol zeta, and provide a mutational signature for mutagenic Pol zeta-dependent activity. In the current study, we have found that a specific subset of the Pol zeta-dependent mutational events requires oxidative metabolism. These results suggest that translesion bypass of spontaneously oxidized DNA bases can be a significant source of mutagenesis in repair compromised cells.
Full Text
Duke Authors
Cited Authors
- Minesinger, BK; Abdulovic, AL; Ou, TM; Jinks-Robertson, S
Published Date
- February 3, 2006
Published In
Volume / Issue
- 5 / 2
Start / End Page
- 226 - 234
PubMed ID
- 16290107
International Standard Serial Number (ISSN)
- 1568-7864
Digital Object Identifier (DOI)
- 10.1016/j.dnarep.2005.10.002
Language
- eng
Conference Location
- Netherlands