The effect of oxidative metabolism on spontaneous Pol zeta-dependent translesion synthesis in Saccharomyces cerevisiae.

Published

Journal Article

DNA lesions can stall or block high-fidelity polymerases, thus inhibiting replication. To bypass such lesions, low-fidelity translesion synthesis (TLS) polymerases can be used to insert a nucleotide across from the lesion or extend from a lesion:base mispair. When DNA repair is compromised in Saccharomyces cerevisiae, spontaneous DNA lesions can lead to a novel mutational event in which a frameshift is accompanied by one or more base pair substitutions. These "complex frameshifts" are dependent upon the TLS polymerase Pol zeta, and provide a mutational signature for mutagenic Pol zeta-dependent activity. In the current study, we have found that a specific subset of the Pol zeta-dependent mutational events requires oxidative metabolism. These results suggest that translesion bypass of spontaneously oxidized DNA bases can be a significant source of mutagenesis in repair compromised cells.

Full Text

Duke Authors

Cited Authors

  • Minesinger, BK; Abdulovic, AL; Ou, TM; Jinks-Robertson, S

Published Date

  • February 3, 2006

Published In

Volume / Issue

  • 5 / 2

Start / End Page

  • 226 - 234

PubMed ID

  • 16290107

Pubmed Central ID

  • 16290107

International Standard Serial Number (ISSN)

  • 1568-7864

Digital Object Identifier (DOI)

  • 10.1016/j.dnarep.2005.10.002

Language

  • eng

Conference Location

  • Netherlands