Genetic requirements for spontaneous and transcription-stimulated mitotic recombination in Saccharomyces cerevisiae.

Published

Journal Article

The genetic requirements for spontaneous and transcription-stimulated mitotic recombination were determined using a recombination system that employs heterochromosomal lys2 substrates that can recombine only by crossover or only by gene conversion. The substrates were fused either to a constitutive low-level promoter (pLYS) or to a highly inducible promoter (pGAL). In the case of the "conversion-only" substrates the use of heterologous promoters allowed either the donor or the recipient allele to be highly transcribed. Transcription of the donor allele stimulated gene conversions in rad50, rad51, rad54, and rad59 mutants, but not in rad52, rad55, and rad57 mutants. In contrast, transcription of the recipient allele stimulated gene conversions in rad50, rad51, rad54, rad55, rad57, and rad59 mutants, but not in rad52 mutants. Finally, transcription stimulated crossovers in rad50, rad54, and rad59 mutants, but not in rad51, rad52, rad55, and rad57 mutants. These data are considered in relation to previously proposed molecular mechanisms of transcription-stimulated recombination and in relation to the roles of the recombination proteins.

Full Text

Duke Authors

Cited Authors

  • Freedman, JA; Jinks-Robertson, S

Published Date

  • September 2002

Published In

Volume / Issue

  • 162 / 1

Start / End Page

  • 15 - 27

PubMed ID

  • 12242220

Pubmed Central ID

  • 12242220

International Standard Serial Number (ISSN)

  • 0016-6731

Language

  • eng

Conference Location

  • United States