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Stimulation of mitotic recombination events by high levels of RNA polymerase II transcription in yeast.

Publication ,  Journal Article
Saxe, D; Datta, A; Jinks-Robertson, S
Published in: Mol Cell Biol
August 2000

The impact of high levels of RNA polymerase II transcription on mitotic recombination was examined using lys2 recombination substrates positioned on nonhomologous chromosomes. Substrates were used that could produce Lys(+) recombinants by either a simple (noncrossover) gene conversion event or a crossover-associated recombination event, by only a simple gene conversion event, or by only a crossover event. Transcription of the lys2 substrates was regulated by the highly inducible GAL1-10 promoter or the low-level LYS2 promoter, with GAL1-10 promoter activity being controlled by the presence or absence of the Gal80p negative regulatory protein. Transcription was found to stimulate recombination in all assays used, but the level of stimulation varied depending on whether only one or both substrates were highly transcribed. In addition, there was an asymmetry in the types of recombination events observed when one substrate versus the other was highly transcribed. Finally, the lys2 substrates were positioned as direct repeats on the same chromosome and were found to exhibit a different recombinational response to high levels of transcription from that exhibited by the repeats on nonhomologous chromosomes. The relevance of these results to the mechanisms of transcription-associated recombination are discussed.

Duke Scholars

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

August 2000

Volume

20

Issue

15

Start / End Page

5404 / 5414

Location

United States

Related Subject Headings

  • Yeasts
  • Transcription, Genetic
  • Recombination, Genetic
  • RNA Polymerase II
  • Promoter Regions, Genetic
  • Mitosis
  • L-Aminoadipate-Semialdehyde Dehydrogenase
  • Genetic Techniques
  • Gene Conversion
  • Developmental Biology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Saxe, D., Datta, A., & Jinks-Robertson, S. (2000). Stimulation of mitotic recombination events by high levels of RNA polymerase II transcription in yeast. Mol Cell Biol, 20(15), 5404–5414. https://doi.org/10.1128/MCB.20.15.5404-5414.2000
Saxe, D., A. Datta, and S. Jinks-Robertson. “Stimulation of mitotic recombination events by high levels of RNA polymerase II transcription in yeast.Mol Cell Biol 20, no. 15 (August 2000): 5404–14. https://doi.org/10.1128/MCB.20.15.5404-5414.2000.
Saxe D, Datta A, Jinks-Robertson S. Stimulation of mitotic recombination events by high levels of RNA polymerase II transcription in yeast. Mol Cell Biol. 2000 Aug;20(15):5404–14.
Saxe, D., et al. “Stimulation of mitotic recombination events by high levels of RNA polymerase II transcription in yeast.Mol Cell Biol, vol. 20, no. 15, Aug. 2000, pp. 5404–14. Pubmed, doi:10.1128/MCB.20.15.5404-5414.2000.
Saxe D, Datta A, Jinks-Robertson S. Stimulation of mitotic recombination events by high levels of RNA polymerase II transcription in yeast. Mol Cell Biol. 2000 Aug;20(15):5404–5414.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

August 2000

Volume

20

Issue

15

Start / End Page

5404 / 5414

Location

United States

Related Subject Headings

  • Yeasts
  • Transcription, Genetic
  • Recombination, Genetic
  • RNA Polymerase II
  • Promoter Regions, Genetic
  • Mitosis
  • L-Aminoadipate-Semialdehyde Dehydrogenase
  • Genetic Techniques
  • Gene Conversion
  • Developmental Biology