Genetic analysis of transcription-associated mutation in Saccharomyces cerevisiae.

Journal Article (Journal Article)

High levels of transcription are associated with elevated mutation rates in yeast, a phenomenon referred to as transcription-associated mutation (TAM). The transcription-associated increase in mutation rates was previously shown to be partially dependent on the Rev3p translesion bypass pathway, thus implicating DNA damage in TAM. In this study, we use reversion of a pGAL-driven lys2DeltaBgl allele to further examine the genetic requirements of TAM. We find that TAM is increased by disruption of the nucleotide excision repair or recombination pathways. In contrast, elimination of base excision repair components has only modest effects on TAM. In addition to the genetic studies, the lys2DeltaBgl reversion spectra of repair-proficient low and high transcription strains were obtained. In the low transcription spectrum, most of the frameshift events correspond to deletions of AT base pairs whereas in the high transcription strain, deletions of GC base pairs predominate. These results are discussed in terms of transcription and its role in DNA damage and repair.

Full Text

Duke Authors

Cited Authors

  • Morey, NJ; Greene, CN; Jinks-Robertson, S

Published Date

  • January 2000

Published In

Volume / Issue

  • 154 / 1

Start / End Page

  • 109 - 120

PubMed ID

  • 10628973

Pubmed Central ID

  • PMC1460922

International Standard Serial Number (ISSN)

  • 0016-6731

Digital Object Identifier (DOI)

  • 10.1093/genetics/154.1.109


  • eng

Conference Location

  • United States