Association of increased spontaneous mutation rates with high levels of transcription in yeast.
Journal Article (Journal Article)
Complex processes such as transcription, replication, repair, and recombination require changes in chromatin structure and the interactions of numerous trans-acting factors with DNA sequences, raising the possibility that these processes may be interrelated. Here the effect of transcription on the rate of spontaneous mutation in the yeast Saccharomyces cerevisiae was examined. With the use of a lys2 frameshift allele under the control of a highly inducible promoter, the rate of spontaneous reversion was shown to increase when the mutant gene was highly transcribed. Thus, transcriptionally active DNA and enhanced spontaneous mutation rates are associated in yeast.
Full Text
Duke Authors
Cited Authors
- Datta, A; Jinks-Robertson, S
Published Date
- June 16, 1995
Published In
Volume / Issue
- 268 / 5217
Start / End Page
- 1616 - 1619
PubMed ID
- 7777859
International Standard Serial Number (ISSN)
- 0036-8075
Digital Object Identifier (DOI)
- 10.1126/science.7777859
Language
- eng
Conference Location
- United States