Substrate length requirements for efficient mitotic recombination in Saccharomyces cerevisiae.

Published

Journal Article

An ectopic recombination system using ura3 heteroalleles varying in size from 80 to 960 bp has been used to examine the effect of substrate length on spontaneous mitotic recombination. The ura3 heteroalleles were positioned either on nonhomologous chromosomes (heterochromosomal repeats) or as direct or inverted repeats on the same chromosome (intrachromosomal repeats). While the intrachromosomal events occur at rates at least 2 orders of magnitude greater than the corresponding heterochromosomal events, the recombination rate for each type of repeat considered separately exhibits a linear dependence on substrate length. The linear relationships allow estimation of the corresponding minimal efficient processing segments, which are approximately 250 bp regardless of the relative positions of the repeats in the yeast genome. An examination of the distribution of recombination events into simple gene conversion versus crossover events indicates that reciprocal exchange is more sensitive to substrate size than is gene conversion.

Full Text

Duke Authors

Cited Authors

  • Jinks-Robertson, S; Michelitch, M; Ramcharan, S

Published Date

  • July 1993

Published In

Volume / Issue

  • 13 / 7

Start / End Page

  • 3937 - 3950

PubMed ID

  • 8321201

Pubmed Central ID

  • 8321201

Electronic International Standard Serial Number (EISSN)

  • 1098-5549

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.13.7.3937

Language

  • eng