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An examination of adaptive reversion in Saccharomyces cerevisiae.

Publication ,  Journal Article
Steele, DF; Jinks-Robertson, S
Published in: Genetics
September 1992

Reversion to Lys+ prototrophy in a haploid yeast strain containing a defined lys2 frameshift mutation has been examined. When cells were plated on synthetic complete medium lacking only lysine, the numbers of Lys+ revertant colonies accumulated in a time-dependent manner in the absence of any detectable increase in cell number. An examination of the distribution of the numbers of early appearing Lys+ colonies from independent cultures suggests that the mutations to prototrophy occurred randomly during nonselective growth. In contrast, an examination of the distribution of late appearing Lys+ colonies indicates that the underlying reversion events occurred after selective plating. No accumulation of Lys+ revertants occurred when cells were starved for tryptophan, leucine or both lysine and tryptophan prior to plating selectively for Lys+ revertants. These results indicate that mutations accumulate more frequently when they confer a selective advantage, and are thus consistent with the occurrence of adaptive mutations in yeast.

Duke Scholars

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

September 1992

Volume

132

Issue

1

Start / End Page

9 / 21

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Transformation, Genetic
  • Saccharomyces cerevisiae
  • Plasmids
  • Mutation
  • Lysine
  • Frameshift Mutation
  • Developmental Biology
  • Culture Media
  • Adaptation, Biological
 

Citation

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ICMJE
MLA
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Steele, D. F., & Jinks-Robertson, S. (1992). An examination of adaptive reversion in Saccharomyces cerevisiae. Genetics, 132(1), 9–21. https://doi.org/10.1093/genetics/132.1.9
Steele, D. F., and S. Jinks-Robertson. “An examination of adaptive reversion in Saccharomyces cerevisiae.Genetics 132, no. 1 (September 1992): 9–21. https://doi.org/10.1093/genetics/132.1.9.
Steele DF, Jinks-Robertson S. An examination of adaptive reversion in Saccharomyces cerevisiae. Genetics. 1992 Sep;132(1):9–21.
Steele, D. F., and S. Jinks-Robertson. “An examination of adaptive reversion in Saccharomyces cerevisiae.Genetics, vol. 132, no. 1, Sept. 1992, pp. 9–21. Pubmed, doi:10.1093/genetics/132.1.9.
Steele DF, Jinks-Robertson S. An examination of adaptive reversion in Saccharomyces cerevisiae. Genetics. 1992 Sep;132(1):9–21.

Published In

Genetics

DOI

ISSN

0016-6731

Publication Date

September 1992

Volume

132

Issue

1

Start / End Page

9 / 21

Location

United States

Related Subject Headings

  • Ultraviolet Rays
  • Transformation, Genetic
  • Saccharomyces cerevisiae
  • Plasmids
  • Mutation
  • Lysine
  • Frameshift Mutation
  • Developmental Biology
  • Culture Media
  • Adaptation, Biological