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Decreased beta-adrenergic responsiveness following hypertrophy occurs only in cardiomyocytes that also re-express beta-myosin heavy chain.

Publication ,  Journal Article
Pandya, K; Porter, K; Rockman, HA; Smithies, O
Published in: Eur J Heart Fail
July 2009

AIMS: Cardiac hypertrophy is associated with a reduction in the contractile response to beta-adrenergic stimulation, and with re-expression of foetal genes such as beta-myosin heavy chain (MHC). However, whether these two markers of pathology develop concordantly in the same individual cells or independently in different cells is not known. METHODS AND RESULTS: To answer this question, we examined the beta-adrenergic response of individual beta-MHC expressing and non-expressing myocytes from hypertrophic hearts, using a previously generated mouse model (YFP/beta-MHC) in which a yellow fluorescent protein (YFP) is fused to the native beta-MHC protein allowing easy identification of beta-MHC expressing cells. Yellow fluorescent protein/beta-MHC mice were submitted to 4 weeks of transverse aortic constriction (TAC), and the contractile parameters of isolated individual myocytes in response to the beta-adrenergic agonist isoproterenol were assessed. Our results demonstrate that the decrease in isoproterenol-induced cell shortening that develops in TAC hearts occurs only in those hypertrophic myocytes that re-express beta-MHC. Hypertrophic myocytes that do not express beta-MHC have contractility indices indistinguishable from non-TAC controls. CONCLUSION: These data show that the reduction of beta-adrenergic response occurs only in subsets, rather than in all myocytes, and is coincident with re-expression of beta-MHC.

Duke Scholars

Published In

Eur J Heart Fail

DOI

ISSN

1388-9842

Publication Date

July 2009

Volume

11

Issue

7

Start / End Page

648 / 652

Location

England

Related Subject Headings

  • Ultrasonography
  • Receptors, Adrenergic, beta
  • Myosin Heavy Chains
  • Myocardium
  • Myocardial Contraction
  • Mice
  • Isoproterenol
  • Disease Models, Animal
  • Cardiovascular System & Hematology
  • Cardiomyopathies
 

Citation

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Pandya, K., Porter, K., Rockman, H. A., & Smithies, O. (2009). Decreased beta-adrenergic responsiveness following hypertrophy occurs only in cardiomyocytes that also re-express beta-myosin heavy chain. Eur J Heart Fail, 11(7), 648–652. https://doi.org/10.1093/eurjhf/hfp073
Pandya, Kumar, Kristine Porter, Howard A. Rockman, and Oliver Smithies. “Decreased beta-adrenergic responsiveness following hypertrophy occurs only in cardiomyocytes that also re-express beta-myosin heavy chain.Eur J Heart Fail 11, no. 7 (July 2009): 648–52. https://doi.org/10.1093/eurjhf/hfp073.
Pandya, Kumar, et al. “Decreased beta-adrenergic responsiveness following hypertrophy occurs only in cardiomyocytes that also re-express beta-myosin heavy chain.Eur J Heart Fail, vol. 11, no. 7, July 2009, pp. 648–52. Pubmed, doi:10.1093/eurjhf/hfp073.
Journal cover image

Published In

Eur J Heart Fail

DOI

ISSN

1388-9842

Publication Date

July 2009

Volume

11

Issue

7

Start / End Page

648 / 652

Location

England

Related Subject Headings

  • Ultrasonography
  • Receptors, Adrenergic, beta
  • Myosin Heavy Chains
  • Myocardium
  • Myocardial Contraction
  • Mice
  • Isoproterenol
  • Disease Models, Animal
  • Cardiovascular System & Hematology
  • Cardiomyopathies