Pulsed-field gel electrophoresis of genomic digests of Thermus strains and its implications for taxonomic and evolutionary studies


Journal Article

Pulsed-field electrophoresis (PFGE) of the SspI genomic digests of 14 Thermus isolates showed that each one had a unique restriction enzyme digestion pattern. A group of New Zealand strains showed some shared bands, but each isolate gave essentially a unique fingerprint. In addition, evolutionary distances between Thermus strains estimated by using PFGE restriction fragment length polymorphisms (PFGE-RFLPs) correlate well with those based on small-subunit rRNA sequence data. As a consequence, the phylogenetic trees constructed on the basis of PFGE-RFLPs and those constructed by using small-subunit rRNA sequences generally agree. On the basis of the evolutionary distances estimated by using PFGE-RFLPs, the estimated average genomic rate of divergence for Thermus spp. is approximately 0.27% per million years.

Full Text

Duke Authors

Cited Authors

  • Rodrigo, AG; Borges, KM; Bergquist, PL

Published Date

  • January 1, 1994

Published In

Volume / Issue

  • 44 / 3

Start / End Page

  • 547 - 552

International Standard Serial Number (ISSN)

  • 0020-7713

Digital Object Identifier (DOI)

  • 10.1099/00207713-44-3-547

Citation Source

  • Scopus