Dynamic regulation of sarcoplasmic reticulum Ca(2+) stores by stromal interaction molecule 1 and sarcolipin during muscle differentiation.

Journal Article (Journal Article)

During muscle development, the sarco/endoplasmic reticulum (SR/ER) undergoes remodeling to establish a specialized internal Ca(2+) store for muscle contraction. We hypothesized that store operated Ca(2+) entry (SOCE) is required to fill Ca(2+) stores and is, therefore, critical to creating a mature SR/ER. Stromal interaction molecule 1 (STIM1) functions as a sensor of internal Ca(2+) store content and an activator of SOCE channels. Myocytes lacking STIM1 display reduced SR Ca(2+) content and altered expression of key SR proteins. Sarcolipin (SLN), an inhibitor of the SR calcium pump, was markedly increased in the muscle of mutant STIM1 mice. SLN opposes the actions of STIM1 by limiting SOCE, reducing SR Ca(2+) content and delaying muscle differentiation. During mouse muscle development SLN is highly expressed in embryonic muscle, while the expression of STIM1 is up-regulated postnatally. These results suggest that SOCE regulates SR/ER specialization and that SLN and STIM1 act in opposing fashions to govern SOCE during myogenesis.

Full Text

Duke Authors

Cited Authors

  • Seth, M; Li, T; Graham, V; Burch, J; Finch, E; Stiber, JA; Rosenberg, PB

Published Date

  • April 2012

Published In

Volume / Issue

  • 241 / 4

Start / End Page

  • 639 - 647

PubMed ID

  • 22411552

Pubmed Central ID

  • PMC3306055

Electronic International Standard Serial Number (EISSN)

  • 1097-0177

Digital Object Identifier (DOI)

  • 10.1002/dvdy.23760


  • eng

Conference Location

  • United States