Autoimmune dacryoadenitis of NOD/LtJ mice and its subsequent effects on tear protein composition.
Journal Article (Journal Article)
Sjögren's syndrome (SjS) is a human autoimmune disease characterized by exocrine dysfunction resulting from chronic autoimmune attack primarily against the lacrimal and/or salivary glands. Although, we previously established a good correlation between SjS in humans and autoimmune exocrinopathy in NOD/LtJ mice an in-depth evaluation of lacrimal gland disease in the NOD/LtJ mouse has remained limited. This leaves a major gap in our understanding of the dacryoadenitis/keratoconjunctivitis sicca in this model. Here we characterize the development of the autoimmune dacryoadenitis in NOD/LtJ and NOD.B10-H2(b) mice in comparison with age- and sex-matched nonautoimmune CD1 mice. We observed a decline in tear production beginning at 8 weeks of age in both NOD/LtJ and NOD.B10-H2(b) mice, continuing throughout the 40 to 46 weeks studied. This correlated with a quantifiable increase in mixed T- and B-lymphocyte infiltrations in the extraorbital lacrimal glands. In addition, temporal differences in tear protein expression between NOD/LtJ and CD1 mice were identified using two-dimensional gel electrophoresis and tandem mass spectrometry. Thus, using this model we can identify potentially important pathophysiological mechanisms of the autoimmune attack and possible diagnostic markers for development of SjS-associated dacryoadenitis.
Full Text
Duke Authors
Cited Authors
- Doyle, ME; Boggs, L; Attia, R; Cooper, LR; Saban, DR; Nguyen, CQ; Peck, AB
Published Date
- October 2007
Published In
Volume / Issue
- 171 / 4
Start / End Page
- 1224 - 1236
PubMed ID
- 17823290
Pubmed Central ID
- PMC1988872
International Standard Serial Number (ISSN)
- 0002-9440
Digital Object Identifier (DOI)
- 10.2353/ajpath.2007.070388
Language
- eng
Conference Location
- United States