Spectral-domain differential interference contrast microscopy.

Journal Article (Journal Article)

We present a fiber-optic low-coherence imaging technique, termed spectral-domain differential interference contrast microscopy (SD-DIC), for quantitative DIC imaging of both reflective surfaces and transparent biological specimens. SD-DIC combines the common-path nature of a Nomarski DIC interferometer with the high sensitivity of spectral-domain low-coherence interferometry to obtain high-resolution, quantitative measurements of optical pathlength gradients from a single point on the sample. Full-field imaging can be achieved by scanning the sample. A reflected-light SD-DIC system was demonstrated using a USAF resolution target as the phase object. Live cardiomyocytes were also imaged, achieving a resolution of 36 pm for pathlength gradient measurements. The dynamics of cardiomyocyte contraction were recorded with high sensitivity at selected sites on the cells.

Full Text

Duke Authors

Cited Authors

  • Zhu, Y; Shaked, NT; Satterwhite, LL; Wax, A

Published Date

  • February 2011

Published In

Volume / Issue

  • 36 / 4

Start / End Page

  • 430 - 432

PubMed ID

  • 21326412

Pubmed Central ID

  • PMC3306060

Electronic International Standard Serial Number (EISSN)

  • 1539-4794

International Standard Serial Number (ISSN)

  • 0146-9592

Digital Object Identifier (DOI)

  • 10.1364/ol.36.000430


  • eng