Skip to main content
Journal cover image

Dynamic adhesion of umbilical cord blood endothelial progenitor cells under laminar shear stress.

Publication ,  Journal Article
Angelos, MG; Brown, MA; Satterwhite, LL; Levering, VW; Shaked, NT; Truskey, GA
Published in: Biophysical journal
December 2010

Late outgrowth endothelial progenitor cells (EPCs) represent a promising cell source for rapid reendothelialization of damaged vasculature after expansion ex vivo and injection into the bloodstream. We characterized the dynamic adhesion of umbilical-cord-blood-derived EPCs (CB-EPCs) to surfaces coated with fibronectin. CB-EPC solution density affected the number of adherent cells and larger cells preferentially adhered at lower cell densities. The number of adherent cells varied with shear stress, with the maximum number of adherent cells and the shear stress at maximum adhesion depending upon fluid viscosity. CB-EPCs underwent limited rolling, transiently tethering for short distances before firm arrest. Immediately before arrest, the instantaneous velocity decreased independent of shear stress. A dimensional analysis indicated that adhesion was a function of the net force on the cells, the ratio of cell diffusion to sliding speed, and molecular diffusivity. Adhesion was not limited by the settling rate and was highly specific to α(5)β(1) integrin. Total internal reflection fluorescence microscopy showed that CB-EPCs produced multiple contacts of α(5)β(1) with the surface and the contact area grew during the first 20 min of attachment. These results demonstrate that CB-EPC adhesion from blood can occur under physiological levels of shear stress.

Duke Scholars

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

December 2010

Volume

99

Issue

11

Start / End Page

3545 / 3554

Related Subject Headings

  • Stress, Mechanical
  • Stem Cells
  • Solutions
  • Receptors, Vitronectin
  • Ligands
  • Humans
  • Fibronectins
  • Fetal Blood
  • Endothelial Cells
  • Diffusion
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Angelos, M. G., Brown, M. A., Satterwhite, L. L., Levering, V. W., Shaked, N. T., & Truskey, G. A. (2010). Dynamic adhesion of umbilical cord blood endothelial progenitor cells under laminar shear stress. Biophysical Journal, 99(11), 3545–3554. https://doi.org/10.1016/j.bpj.2010.10.004
Angelos, Mathew G., Melissa A. Brown, Lisa L. Satterwhite, Vrad W. Levering, Natan T. Shaked, and George A. Truskey. “Dynamic adhesion of umbilical cord blood endothelial progenitor cells under laminar shear stress.Biophysical Journal 99, no. 11 (December 2010): 3545–54. https://doi.org/10.1016/j.bpj.2010.10.004.
Angelos MG, Brown MA, Satterwhite LL, Levering VW, Shaked NT, Truskey GA. Dynamic adhesion of umbilical cord blood endothelial progenitor cells under laminar shear stress. Biophysical journal. 2010 Dec;99(11):3545–54.
Angelos, Mathew G., et al. “Dynamic adhesion of umbilical cord blood endothelial progenitor cells under laminar shear stress.Biophysical Journal, vol. 99, no. 11, Dec. 2010, pp. 3545–54. Epmc, doi:10.1016/j.bpj.2010.10.004.
Angelos MG, Brown MA, Satterwhite LL, Levering VW, Shaked NT, Truskey GA. Dynamic adhesion of umbilical cord blood endothelial progenitor cells under laminar shear stress. Biophysical journal. 2010 Dec;99(11):3545–3554.
Journal cover image

Published In

Biophysical journal

DOI

EISSN

1542-0086

ISSN

0006-3495

Publication Date

December 2010

Volume

99

Issue

11

Start / End Page

3545 / 3554

Related Subject Headings

  • Stress, Mechanical
  • Stem Cells
  • Solutions
  • Receptors, Vitronectin
  • Ligands
  • Humans
  • Fibronectins
  • Fetal Blood
  • Endothelial Cells
  • Diffusion