Quality control and reference guidelines for CLSI broth microdilution method (M38-A document) for susceptibility testing of anidulafungin against molds.

Journal Article (Journal Article;Multicenter Study)

The CLSI (formerly NCCLS) M38-A document for antifungal susceptibility testing of filamentous fungi does not describe guidelines for echinocandins. A multicenter study (eight centers) evaluated inter- and intralaboratory reproducibilities of two reading times (24 and 48 h or 48 and 72 h) and two end points (MICs and minimum effective concentrations [MECs]) for evaluating anidulafungin against molds. Anidulafungin MICs (>or=50% inhibition) and MECs (morphological hyphal changes) were determined for seven Aspergillus isolates (four species) and one isolate each of Fusarium moniliforme, Fusarium solani, and Paecilomyces variotii and for two Scedosporium apiospermum isolates. The inter- and intralaboratory reproducibilities of 10 replicate tests performed in each laboratory on 10 different days for each isolate was 100% at 24 h (MECs, or=32 microg/ml) for both Fusarium isolates. Introduction of these identified optimum testing conditions for anidulafungin into future versions of the M38 document is warranted.

Full Text

Duke Authors

Cited Authors

  • Espinel-Ingroff, A; Fothergill, A; Ghannoum, M; Manavathu, E; Ostrosky-Zeichner, L; Pfaller, MA; Rinaldi, MG; Schell, W; Walsh, TJ

Published Date

  • July 2007

Published In

Volume / Issue

  • 45 / 7

Start / End Page

  • 2180 - 2182

PubMed ID

  • 17475760

Pubmed Central ID

  • PMC1932988

International Standard Serial Number (ISSN)

  • 0095-1137

Digital Object Identifier (DOI)

  • 10.1128/JCM.00399-07


  • eng

Conference Location

  • United States