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Quality control and reference guidelines for CLSI broth microdilution method (M38-A document) for susceptibility testing of anidulafungin against molds.

Publication ,  Journal Article
Espinel-Ingroff, A; Fothergill, A; Ghannoum, M; Manavathu, E; Ostrosky-Zeichner, L; Pfaller, MA; Rinaldi, MG; Schell, W; Walsh, TJ
Published in: J Clin Microbiol
July 2007

The CLSI (formerly NCCLS) M38-A document for antifungal susceptibility testing of filamentous fungi does not describe guidelines for echinocandins. A multicenter study (eight centers) evaluated inter- and intralaboratory reproducibilities of two reading times (24 and 48 h or 48 and 72 h) and two end points (MICs and minimum effective concentrations [MECs]) for evaluating anidulafungin against molds. Anidulafungin MICs (>or=50% inhibition) and MECs (morphological hyphal changes) were determined for seven Aspergillus isolates (four species) and one isolate each of Fusarium moniliforme, Fusarium solani, and Paecilomyces variotii and for two Scedosporium apiospermum isolates. The inter- and intralaboratory reproducibilities of 10 replicate tests performed in each laboratory on 10 different days for each isolate was 100% at 24 h (MECs, or=32 microg/ml) for both Fusarium isolates. Introduction of these identified optimum testing conditions for anidulafungin into future versions of the M38 document is warranted.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

July 2007

Volume

45

Issue

7

Start / End Page

2180 / 2182

Location

United States

Related Subject Headings

  • Reproducibility of Results
  • Quality Control
  • Peptides, Cyclic
  • Microbiology
  • Microbial Sensitivity Tests
  • Laboratories
  • Guidelines as Topic
  • Fungi
  • Echinocandins
  • Drug Resistance, Fungal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Espinel-Ingroff, A., Fothergill, A., Ghannoum, M., Manavathu, E., Ostrosky-Zeichner, L., Pfaller, M. A., … Walsh, T. J. (2007). Quality control and reference guidelines for CLSI broth microdilution method (M38-A document) for susceptibility testing of anidulafungin against molds. J Clin Microbiol, 45(7), 2180–2182. https://doi.org/10.1128/JCM.00399-07
Espinel-Ingroff, A., A. Fothergill, M. Ghannoum, E. Manavathu, L. Ostrosky-Zeichner, M. A. Pfaller, M. G. Rinaldi, W. Schell, and T. J. Walsh. “Quality control and reference guidelines for CLSI broth microdilution method (M38-A document) for susceptibility testing of anidulafungin against molds.J Clin Microbiol 45, no. 7 (July 2007): 2180–82. https://doi.org/10.1128/JCM.00399-07.
Espinel-Ingroff A, Fothergill A, Ghannoum M, Manavathu E, Ostrosky-Zeichner L, Pfaller MA, et al. Quality control and reference guidelines for CLSI broth microdilution method (M38-A document) for susceptibility testing of anidulafungin against molds. J Clin Microbiol. 2007 Jul;45(7):2180–2.
Espinel-Ingroff, A., et al. “Quality control and reference guidelines for CLSI broth microdilution method (M38-A document) for susceptibility testing of anidulafungin against molds.J Clin Microbiol, vol. 45, no. 7, July 2007, pp. 2180–82. Pubmed, doi:10.1128/JCM.00399-07.
Espinel-Ingroff A, Fothergill A, Ghannoum M, Manavathu E, Ostrosky-Zeichner L, Pfaller MA, Rinaldi MG, Schell W, Walsh TJ. Quality control and reference guidelines for CLSI broth microdilution method (M38-A document) for susceptibility testing of anidulafungin against molds. J Clin Microbiol. 2007 Jul;45(7):2180–2182.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

July 2007

Volume

45

Issue

7

Start / End Page

2180 / 2182

Location

United States

Related Subject Headings

  • Reproducibility of Results
  • Quality Control
  • Peptides, Cyclic
  • Microbiology
  • Microbial Sensitivity Tests
  • Laboratories
  • Guidelines as Topic
  • Fungi
  • Echinocandins
  • Drug Resistance, Fungal