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Molecular characterization of inflammatory genes in sentinel and nonsentinel nodes in melanoma.

Publication ,  Journal Article
Torisu-Itakura, H; Lee, JH; Scheri, RP; Huynh, Y; Ye, X; Essner, R; Morton, DL
Published in: Clin Cancer Res
June 1, 2007

PURPOSE: Identification of regional node metastasis is important for accurate staging and optimal treatment of early melanoma. We hypothesize that the nodal profile of immunoregulatory cytokines can confirm the identity of the first tumor-draining regional node, i.e., the sentinel node (SN) and indicate its tumor status. EXPERIMENTAL DESIGN: RNA was extracted from freshly dissected and preserved nodal tissue of 13 tumor-negative SNs, 10 tumor-positive SNs (micrometastases <2 mm), and 11 tumor-negative non-SNs (NSN). RNA was converted into cDNA and then amplified by PCR. Expression of 96 cytokines and chemokines was assessed using cDNA microarray and compared by using hierarchical clustering. RESULTS: Fifty-seven genes were expressed at significantly (P < 0.05) different levels in SNs and NSNs (4 genes had higher expression, and 53 genes had lower expression in SNs). Expression levels of interleukin-13 (IL-13), leptin, lymphotoxin beta receptor (LTbR), and macrophage inflammatory protein 1b (MIP1b) were significantly higher (P < 0.04, P < 0.01, P < 0.05, and P < 0.01, respectively), and expression level of IL-11Ra was lower (P < 0.03) for tumor-positive as compared with tumor-negative SN. Receiver-operator characteristics curve analyses showed that the area under the curve (AUC) for IL-13, leptin, LTbR, MIP1b, and IL-11Ra was 0.79, 0.83, 0.75, 0.81, and 0.77, respectively. The AUC for the five genes in combination was 0.973, suggesting high concordance of gene-expression profiles with SN staging. CONCLUSIONS: SNs have a different immunoregulatory cytokine profile than NSNs. The cytokine profile of tumor-positive SNs; increased expression of IL-13, leptin, LTbR, and MIP1b and decreased expression of IL-11Ra, may provide clues to the local tumor lymph node interaction seen in the earliest steps of melanoma metastasis.

Duke Scholars

Published In

Clin Cancer Res

DOI

ISSN

1078-0432

Publication Date

June 1, 2007

Volume

13

Issue

11

Start / End Page

3125 / 3132

Location

United States

Related Subject Headings

  • Skin Neoplasms
  • Sentinel Lymph Node Biopsy
  • Receptors, Interleukin-11
  • Oncology & Carcinogenesis
  • Neoplasm Metastasis
  • Melanoma
  • Lymph Nodes
  • Leptin
  • Interleukin-13
  • Inflammation
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Torisu-Itakura, H., Lee, J. H., Scheri, R. P., Huynh, Y., Ye, X., Essner, R., & Morton, D. L. (2007). Molecular characterization of inflammatory genes in sentinel and nonsentinel nodes in melanoma. Clin Cancer Res, 13(11), 3125–3132. https://doi.org/10.1158/1078-0432.CCR-06-2645
Torisu-Itakura, Hitoe, Jonathan H. Lee, Randall P. Scheri, Young Huynh, Xing Ye, Richard Essner, and Donald L. Morton. “Molecular characterization of inflammatory genes in sentinel and nonsentinel nodes in melanoma.Clin Cancer Res 13, no. 11 (June 1, 2007): 3125–32. https://doi.org/10.1158/1078-0432.CCR-06-2645.
Torisu-Itakura H, Lee JH, Scheri RP, Huynh Y, Ye X, Essner R, et al. Molecular characterization of inflammatory genes in sentinel and nonsentinel nodes in melanoma. Clin Cancer Res. 2007 Jun 1;13(11):3125–32.
Torisu-Itakura, Hitoe, et al. “Molecular characterization of inflammatory genes in sentinel and nonsentinel nodes in melanoma.Clin Cancer Res, vol. 13, no. 11, June 2007, pp. 3125–32. Pubmed, doi:10.1158/1078-0432.CCR-06-2645.
Torisu-Itakura H, Lee JH, Scheri RP, Huynh Y, Ye X, Essner R, Morton DL. Molecular characterization of inflammatory genes in sentinel and nonsentinel nodes in melanoma. Clin Cancer Res. 2007 Jun 1;13(11):3125–3132.

Published In

Clin Cancer Res

DOI

ISSN

1078-0432

Publication Date

June 1, 2007

Volume

13

Issue

11

Start / End Page

3125 / 3132

Location

United States

Related Subject Headings

  • Skin Neoplasms
  • Sentinel Lymph Node Biopsy
  • Receptors, Interleukin-11
  • Oncology & Carcinogenesis
  • Neoplasm Metastasis
  • Melanoma
  • Lymph Nodes
  • Leptin
  • Interleukin-13
  • Inflammation