Structure and function of the native and recombinant mitochondrial MRP1/MRP2 complex from Trypanosoma brucei.

Published

Journal Article

The mitochondrial RNA-binding proteins (MRP) 1 and 2 play a regulatory role in RNA editing and putative role(s) in RNA processing in Trypanosoma brucei. Here, we report the purification of a high molecular weight protein complex consisting solely of the MRP1 and MRP2 proteins from the mitochondrion of T. brucei. The MRP1/MRP2 complex natively purified from T. brucei and the one reconstituted in Escherichia coli in vivo bind guide (g) RNAs and pre-mRNAs with dissociation constants in the nanomolar range, and efficiently promote annealing of pre-mRNAs with their cognate gRNAs. In addition, the MRP1/MRP2 complex stimulates annealing between two non-cognate RNA molecules suggesting that along with the cognate duplexes, spuriously mismatched RNA hybrids may be formed at some rate in vivo. A mechanism of catalysed annealing of gRNA/pre-mRNA by the MRP1/MRP2 complex is proposed.

Full Text

Duke Authors

Cited Authors

  • Zíková, A; Kopecná, J; Schumacher, MA; Stuart, K; Trantírek, L; Lukes, J

Published Date

  • July 2008

Published In

Volume / Issue

  • 38 / 8-9

Start / End Page

  • 901 - 912

PubMed ID

  • 18295767

Pubmed Central ID

  • 18295767

International Standard Serial Number (ISSN)

  • 0020-7519

Digital Object Identifier (DOI)

  • 10.1016/j.ijpara.2007.12.009

Language

  • eng

Conference Location

  • England