Structural basis of core promoter recognition in a primitive eukaryote.

Published

Journal Article

Transcription start site selection in eukaryotes is mediated through combinations of the TATA, initiator (Inr), and downstream promoter elements (DPE). In Trichomonas vaginalis, a parabasalian flagellate thought to represent an ancient eukaryote lineage, the Inr appears to be solely responsible for start site selection and is recognized by the initiator binding protein 39 kDa (IBP39). IBP39 contains an N-terminal Inr binding domain (IBD) connected via a flexible linker to a C-terminal domain (C domain). Here we present crystal structures of the apoIBD and IBD-Inr complexes and the C domain. The IBD structures reveal a winged-helix motif with prokaryotic and eukaryotic features and a scaffold similar to that of ETS-family proteins. The C domain structure and biochemical studies indicate that it interacts with the T. vaginalis RNAP II large subunit C-terminal domain. These data suggest that binding of IBP39 to the Inr directly recruits RNAP II and in this way initiates transcription.

Full Text

Duke Authors

Cited Authors

  • Schumacher, MA; Lau, AOT; Johnson, PJ

Published Date

  • November 14, 2003

Published In

Volume / Issue

  • 115 / 4

Start / End Page

  • 413 - 424

PubMed ID

  • 14622596

Pubmed Central ID

  • 14622596

International Standard Serial Number (ISSN)

  • 0092-8674

Language

  • eng

Conference Location

  • United States