The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe.

Published online

Journal Article

A strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, we found that polyadenylated antisense transcripts are broadly expressed while distinct expression patterns were observed for protein-coding and non-coding loci. Dominant antisense expression is enriched in protein-coding genes involved in meiosis or stress response pathways. Detailed analyses further suggest that antisense transcripts are independently regulated with respect to their sense transcripts, and diverse mechanisms might be potentially involved in the biogenesis and degradation of antisense RNAs. Taken together, antisense transcription may have profound impacts on global gene regulation in S. pombe.

Full Text

Duke Authors

Cited Authors

  • Ni, T; Tu, K; Wang, Z; Song, S; Wu, H; Xie, B; Scott, KC; Grewal, SI; Gao, Y; Zhu, J

Published Date

  • December 20, 2010

Published In

Volume / Issue

  • 5 / 12

Start / End Page

  • e15271 -

PubMed ID

  • 21187966

Electronic International Standard Serial Number (EISSN)

  • 1932-6203

Digital Object Identifier (DOI)

  • 10.1371/journal.pone.0015271

Language

  • eng

Conference Location

  • United States