Anabolic function of the type II isozyme of hexokinase in hepatic lipid synthesis.

Published

Journal Article

The mRNA encoding the Type II isozyme of hexokinase was markedly elevated in livers of transgenic mice overexpressing the transcriptionally active nuclear form of sterol regulatory element binding protein-1a (nSREBP-1a), but not in transgenic mice overexpressing the nSREBP-1c or nSREBP-2 isoforms. Cellulose acetate electrophoresis and immunoblotting results confirmed selective increase of the Type II isozyme in livers of transgenic mice expressing nSREBP-1a. SREBP-1a has previously been shown to activate transcription of genes encoding enzymes involved in biosynthesis of fatty acids and glycerolipids and to a lesser extent the enzymes of cholesterol biosynthesis. Thus, these results are consistent with the view that the Type II isozyme serves an anabolic function, providing precursors and reducing equivalents required for increased rates of hepatic lipid synthesis.

Full Text

Duke Authors

Cited Authors

  • Sebastian, S; Horton, JD; Wilson, JE

Published Date

  • April 21, 2000

Published In

Volume / Issue

  • 270 / 3

Start / End Page

  • 886 - 891

PubMed ID

  • 10772920

Pubmed Central ID

  • 10772920

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1006/bbrc.2000.2527

Language

  • eng

Conference Location

  • United States