Stimulation of brain hexokinase gene expression by recombinant brain insulin-like growth factor in C6 glial cells.
Journal Article (Journal Article)
Glycolysis is essential for cerebral energy generation. Hence, expression and regulation of gene-encoding brain hexokinase (HK I), the exclusive brain glucose phosphorylating enzyme, can be a critical step in this process. The present study demonstrates the ability of recombinant brain insulin-like growth factor (BIGF, a closely related member of insulin superfamily) to stimulate HK I gene expression in a concentration- and time-dependent manner in C6 glial cells. BIGF treatment (10 ng/ml) on quiescent C6 glial cells stimulates transcription and translation of HK I RNA to approximately 2.5-fold within 4 h after the addition of growth factor. In contrast, insulin or epidermal growth factor could not mimic this effect. Coincubation of cycloheximide with BIGF abolished this stimulatory effect, indicating a requirement for prior protein synthesis for this effect. These results suggest that IGF may have a role in regulating hexokinase gene expression in brain and possibly of brain glucose metabolism.
Full Text
Duke Authors
Cited Authors
- Sebastian, S; Kenkare, UW
Published Date
- January 10, 1999
Published In
Volume / Issue
- 246 / 1
Start / End Page
- 243 - 247
PubMed ID
- 9882533
International Standard Serial Number (ISSN)
- 0014-4827
Digital Object Identifier (DOI)
- 10.1006/excr.1998.4281
Language
- eng
Conference Location
- United States