Stimulation of brain hexokinase gene expression by recombinant brain insulin-like growth factor in C6 glial cells.

Published

Journal Article

Glycolysis is essential for cerebral energy generation. Hence, expression and regulation of gene-encoding brain hexokinase (HK I), the exclusive brain glucose phosphorylating enzyme, can be a critical step in this process. The present study demonstrates the ability of recombinant brain insulin-like growth factor (BIGF, a closely related member of insulin superfamily) to stimulate HK I gene expression in a concentration- and time-dependent manner in C6 glial cells. BIGF treatment (10 ng/ml) on quiescent C6 glial cells stimulates transcription and translation of HK I RNA to approximately 2.5-fold within 4 h after the addition of growth factor. In contrast, insulin or epidermal growth factor could not mimic this effect. Coincubation of cycloheximide with BIGF abolished this stimulatory effect, indicating a requirement for prior protein synthesis for this effect. These results suggest that IGF may have a role in regulating hexokinase gene expression in brain and possibly of brain glucose metabolism.

Full Text

Duke Authors

Cited Authors

  • Sebastian, S; Kenkare, UW

Published Date

  • January 10, 1999

Published In

Volume / Issue

  • 246 / 1

Start / End Page

  • 243 - 247

PubMed ID

  • 9882533

Pubmed Central ID

  • 9882533

International Standard Serial Number (ISSN)

  • 0014-4827

Digital Object Identifier (DOI)

  • 10.1006/excr.1998.4281

Language

  • eng

Conference Location

  • United States