Skip to main content

Next-generation sequencing of apoptotic DNA breakpoints reveals association with actively transcribed genes and gene translocations.

Publication ,  Journal Article
Fullwood, MJ; Lee, J; Lin, L; Li, G; Huss, M; Ng, P; Sung, W-K; Shenolikar, S
Published in: PLoS One
2011

DNA fragmentation is a well-recognized hallmark of apoptosis. However, the precise DNA sequences cleaved during apoptosis triggered by distinct mechanisms remain unclear. We used next-generation sequencing of DNA fragments generated in Actinomycin D-treated human HL-60 leukemic cells to generate a high-throughput, global map of apoptotic DNA breakpoints. These data highlighted that DNA breaks are non-random and show a significant association with active genes and open chromatin regions. We noted that transcription factor binding sites were also enriched within a fraction of the apoptotic breakpoints. Interestingly, extensive apoptotic cleavage was noted within genes that are frequently translocated in human cancers. We speculate that the non-random fragmentation of DNA during apoptosis may contribute to gene translocations and the development of human cancers.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2011

Volume

6

Issue

11

Start / End Page

e26054

Location

United States

Related Subject Headings

  • Translocation, Genetic
  • Transcription, Genetic
  • Transcription Factors
  • Humans
  • High-Throughput Nucleotide Sequencing
  • General Science & Technology
  • Dactinomycin
  • DNA Fragmentation
  • Cell Line, Tumor
  • Binding Sites
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Fullwood, M. J., Lee, J., Lin, L., Li, G., Huss, M., Ng, P., … Shenolikar, S. (2011). Next-generation sequencing of apoptotic DNA breakpoints reveals association with actively transcribed genes and gene translocations. PLoS One, 6(11), e26054. https://doi.org/10.1371/journal.pone.0026054
Fullwood, Melissa J., Joanne Lee, Lifang Lin, Guoliang Li, Mikael Huss, Patrick Ng, Wing-Kin Sung, and Shirish Shenolikar. “Next-generation sequencing of apoptotic DNA breakpoints reveals association with actively transcribed genes and gene translocations.PLoS One 6, no. 11 (2011): e26054. https://doi.org/10.1371/journal.pone.0026054.
Fullwood, Melissa J., et al. “Next-generation sequencing of apoptotic DNA breakpoints reveals association with actively transcribed genes and gene translocations.PLoS One, vol. 6, no. 11, 2011, p. e26054. Pubmed, doi:10.1371/journal.pone.0026054.
Fullwood MJ, Lee J, Lin L, Li G, Huss M, Ng P, Sung W-K, Shenolikar S. Next-generation sequencing of apoptotic DNA breakpoints reveals association with actively transcribed genes and gene translocations. PLoS One. 2011;6(11):e26054.

Published In

PLoS One

DOI

EISSN

1932-6203

Publication Date

2011

Volume

6

Issue

11

Start / End Page

e26054

Location

United States

Related Subject Headings

  • Translocation, Genetic
  • Transcription, Genetic
  • Transcription Factors
  • Humans
  • High-Throughput Nucleotide Sequencing
  • General Science & Technology
  • Dactinomycin
  • DNA Fragmentation
  • Cell Line, Tumor
  • Binding Sites