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Defective PTH regulation of sodium-dependent phosphate transport in NHERF-1-/- renal proximal tubule cells and wild-type cells adapted to low-phosphate media.

Publication ,  Journal Article
Cunningham, R; E, X; Steplock, D; Shenolikar, S; Weinman, EJ
Published in: Am J Physiol Renal Physiol
October 2005

The present experiments using primary cultures from renal proximal tubule cells examine two aspects of the regulation of sodium-dependent phosphate transport and membrane sodium-dependent phosphate transporter (Npt2a) expression by parathyroid hormone (PTH). Sodium-dependent phosphate transport in proximal tubule cells from wild-type mice grown in normal-phosphate media averaged 4.4 +/- 0.5 nmol.mg protein(-1).10 min(-1) and was inhibited by 30.5 +/- 8.6% by PTH (10(-7) M). This was associated with a 32.7 +/- 5.2% decrease in Npt2a expression in the plasma membrane. Proximal tubule cells from Na(+)/H(+) exchanger regulatory factor-1 (NHERF-1)(-/-) mice had a lower rate of phosphate transport compared with wild-type cells and a significantly reduced inhibitory response to PTH. Wild-type cells incubated in low-phosphate media for 24 h had a higher rate of phosphate transport compared with wild-type cells grown in normal-phosphate media but a significantly blunted inhibitory response to PTH. These data indicate a role for NHERF-1 in mediating the membrane retrieval of Npt2a and the subsequent inhibition of phosphate transport in renal proximal tubules. These studies also suggest that there is a blunted phosphaturic effect of PTH in cells adapted to low-phosphate media.

Duke Scholars

Published In

Am J Physiol Renal Physiol

DOI

ISSN

1931-857X

Publication Date

October 2005

Volume

289

Issue

4

Start / End Page

F933 / F938

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Sodium-Hydrogen Exchangers
  • Sodium
  • Protein Kinase C
  • Phosphoproteins
  • Phosphates
  • Parathyroid Hormone
  • Mitogen-Activated Protein Kinases
  • Mice, Knockout
  • Mice, Inbred C57BL
 

Citation

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MLA
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Cunningham, R., E, X., Steplock, D., Shenolikar, S., & Weinman, E. J. (2005). Defective PTH regulation of sodium-dependent phosphate transport in NHERF-1-/- renal proximal tubule cells and wild-type cells adapted to low-phosphate media. Am J Physiol Renal Physiol, 289(4), F933–F938. https://doi.org/10.1152/ajprenal.00005.2005
Cunningham, Rochelle, Xiaofei E, Deborah Steplock, Shirish Shenolikar, and Edward J. Weinman. “Defective PTH regulation of sodium-dependent phosphate transport in NHERF-1-/- renal proximal tubule cells and wild-type cells adapted to low-phosphate media.Am J Physiol Renal Physiol 289, no. 4 (October 2005): F933–38. https://doi.org/10.1152/ajprenal.00005.2005.
Cunningham R, E X, Steplock D, Shenolikar S, Weinman EJ. Defective PTH regulation of sodium-dependent phosphate transport in NHERF-1-/- renal proximal tubule cells and wild-type cells adapted to low-phosphate media. Am J Physiol Renal Physiol. 2005 Oct;289(4):F933–8.
Cunningham, Rochelle, et al. “Defective PTH regulation of sodium-dependent phosphate transport in NHERF-1-/- renal proximal tubule cells and wild-type cells adapted to low-phosphate media.Am J Physiol Renal Physiol, vol. 289, no. 4, Oct. 2005, pp. F933–38. Pubmed, doi:10.1152/ajprenal.00005.2005.
Cunningham R, E X, Steplock D, Shenolikar S, Weinman EJ. Defective PTH regulation of sodium-dependent phosphate transport in NHERF-1-/- renal proximal tubule cells and wild-type cells adapted to low-phosphate media. Am J Physiol Renal Physiol. 2005 Oct;289(4):F933–F938.

Published In

Am J Physiol Renal Physiol

DOI

ISSN

1931-857X

Publication Date

October 2005

Volume

289

Issue

4

Start / End Page

F933 / F938

Location

United States

Related Subject Headings

  • Urology & Nephrology
  • Sodium-Hydrogen Exchangers
  • Sodium
  • Protein Kinase C
  • Phosphoproteins
  • Phosphates
  • Parathyroid Hormone
  • Mitogen-Activated Protein Kinases
  • Mice, Knockout
  • Mice, Inbred C57BL