Overview of protein phosphorylation.

Published

Journal Article (Review)

This overview provides a history of protein phosphorylation research and provides the reader with an understanding of how and why labeling studies are performed. The various sites of protein phosphorylation are described along with the roles of the many kinases and phosphatases that regulate phosphorylation. Methods for detecting unlabeled phosphoamino acids, including high-voltage electrophoresis on thin-layer cellulose acetate plates, gel-shift assays, and the use of anti-phosphopeptide antibodies.

Full Text

Duke Authors

Cited Authors

  • Sefton, BM; Shenolikar, S

Published Date

  • May 2001

Published In

Volume / Issue

  • Chapter 13 /

Start / End Page

  • Unit13.1 -

PubMed ID

  • 18429113

Pubmed Central ID

  • 18429113

Electronic International Standard Serial Number (EISSN)

  • 1934-3663

Digital Object Identifier (DOI)

  • 10.1002/0471140864.ps1301s00

Language

  • eng

Conference Location

  • United States