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Overview of protein phosphorylation.

Publication ,  Journal Article
Sefton, BM; Shenolikar, S
Published in: Curr Protoc Protein Sci
May 2001

This overview provides a history of protein phosphorylation research and provides the reader with an understanding of how and why labeling studies are performed. The various sites of protein phosphorylation are described along with the roles of the many kinases and phosphatases that regulate phosphorylation. Methods for detecting unlabeled phosphoamino acids, including high-voltage electrophoresis on thin-layer cellulose acetate plates, gel-shift assays, and the use of anti-phosphopeptide antibodies.

Duke Scholars

Published In

Curr Protoc Protein Sci

DOI

EISSN

1934-3663

Publication Date

May 2001

Volume

Chapter 13

Start / End Page

Unit13.1

Location

United States

Related Subject Headings

  • Proteins
  • Protein-Tyrosine Kinases
  • Phosphorylation
  • Phosphoprotein Phosphatases
  • Phosphoamino Acids
  • Animals
 

Citation

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Sefton, B. M., & Shenolikar, S. (2001). Overview of protein phosphorylation. Curr Protoc Protein Sci, Chapter 13, Unit13.1. https://doi.org/10.1002/0471140864.ps1301s00
Sefton, B. M., and S. Shenolikar. “Overview of protein phosphorylation.Curr Protoc Protein Sci Chapter 13 (May 2001): Unit13.1. https://doi.org/10.1002/0471140864.ps1301s00.
Sefton BM, Shenolikar S. Overview of protein phosphorylation. Curr Protoc Protein Sci. 2001 May;Chapter 13:Unit13.1.
Sefton, B. M., and S. Shenolikar. “Overview of protein phosphorylation.Curr Protoc Protein Sci, vol. Chapter 13, May 2001, p. Unit13.1. Pubmed, doi:10.1002/0471140864.ps1301s00.
Sefton BM, Shenolikar S. Overview of protein phosphorylation. Curr Protoc Protein Sci. 2001 May;Chapter 13:Unit13.1.

Published In

Curr Protoc Protein Sci

DOI

EISSN

1934-3663

Publication Date

May 2001

Volume

Chapter 13

Start / End Page

Unit13.1

Location

United States

Related Subject Headings

  • Proteins
  • Protein-Tyrosine Kinases
  • Phosphorylation
  • Phosphoprotein Phosphatases
  • Phosphoamino Acids
  • Animals