Visualizing G protein-coupled receptor signalsomes using confocal immunofluorescence microscopy.
Journal Article (Journal Article)
The heptahelical G protein-coupled receptors (GPCRs) receive and transmit a wide range of extracellular stimuli and induce a wide array of cellular responses by activating signaling kinases. It has become increasingly evident that the agonist-stimulated GPCR complexed with the adaptor protein, β-arrestin, serves as a focal point to recruit, activate, and target kinases to discrete subcellular compartments. This chapter describes a protocol to visualize the changes in the subcellular distribution of activated extracellular signal-regulated kinases 1 and 2 (ERK1/2) when induced by the angiotensin II type 1a receptor.
Full Text
Duke Authors
Cited Authors
- Shenoy, SK
Published Date
- 2011
Published In
Volume / Issue
- 756 /
Start / End Page
- 333 - 342
PubMed ID
- 21870237
Pubmed Central ID
- 21870237
Electronic International Standard Serial Number (EISSN)
- 1940-6029
Digital Object Identifier (DOI)
- 10.1007/978-1-61779-160-4_20
Language
- eng
Conference Location
- United States