Visualizing G protein-coupled receptor signalsomes using confocal immunofluorescence microscopy.

Journal Article

The heptahelical G protein-coupled receptors (GPCRs) receive and transmit a wide range of extracellular stimuli and induce a wide array of cellular responses by activating signaling kinases. It has become increasingly evident that the agonist-stimulated GPCR complexed with the adaptor protein, β-arrestin, serves as a focal point to recruit, activate, and target kinases to discrete subcellular compartments. This chapter describes a protocol to visualize the changes in the subcellular distribution of activated extracellular signal-regulated kinases 1 and 2 (ERK1/2) when induced by the angiotensin II type 1a receptor.

Full Text

Duke Authors

Cited Authors

  • Shenoy, SK

Published Date

  • 2011

Published In

Volume / Issue

  • 756 /

Start / End Page

  • 333 - 342

PubMed ID

  • 21870237

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-61779-160-4_20

Language

  • eng

Conference Location

  • United States