Rec A-independent homologous recombination induced by a putative fold-back tetraplex DNA.

Published

Journal Article

We have recently reported that a GC-rich palindromic repeat sequence presumably adopts a stable fold-back tetraplex DNA structure under supercoiling. To establish the biological significance of this structure, we inserted this sequence between two direct repeat sequences, separated by 200 bp, in a plasmid. We then investigated the effect of this sequence on homologous recombination events. Here we report that the putative fold-back DNA tetraplex structure induces homologous recombination between direct repeat sequences. Interestingly, this recombination event is independent of recA, a major driving force for homologous recombination. We think that the fold-back structure forces the repeat sequences to come into close proximity and therefore leads to strand exchange. Although triplex-induced recombination has been well documented, our results for the first time directly establish the potential of a tetraplex structure to induce recA-independent homologous recombination in vivo. This finding might have a significant implication for site-directed gene deletion in the context of the correction of genetic defects.

Full Text

Cited Authors

  • Shukla, AK; Roy, KB

Published Date

  • March 2006

Published In

Volume / Issue

  • 387 / 3

Start / End Page

  • 251 - 256

PubMed ID

  • 16542145

Pubmed Central ID

  • 16542145

Electronic International Standard Serial Number (EISSN)

  • 1437-4315

International Standard Serial Number (ISSN)

  • 1431-6730

Digital Object Identifier (DOI)

  • 10.1515/bc.2006.033

Language

  • eng