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Modulation of IA currents by capsaicin in rat trigeminal ganglion neurons.

Publication ,  Journal Article
Liu, L; Simon, SA
Published in: J Neurophysiol
March 2003

When capsaicin, the pungent compound in hot pepper, is applied to epithelia it produces pain, allodynia, and hyperalgesia. We investigated, using whole cell path clamp, whether some of these responses induced by capsaicin could be a consequence of capsaicin blocking I(A) currents, a reduction in which, such as occurs in injury, increases neuronal excitability. In capsaicin-sensitive (CS) rat trigeminal ganglion (TG) neurons, capsaicin inhibited I(A) currents in a dose-dependent manner. I(A) currents were reduced 49% by 1 microM capsaicin. In capsaicin-insensitive (CIS) rat TG neurons, or small-diameter mouse VR1-/- neurons, 1 microM capsaicin inhibited I(A) currents 9 and 3%, respectively. These data suggest that in CS neurons the vast majority of the capsaicin-induced inhibition of I(A) currents occurs as a consequence of the activation of vanilloid receptors. Capsaicin (1 microM) did not alter the I(A) conductance-voltage relationship but shifted the inactivation-voltage curve about 15 mV to hyperpolarizing voltages, thereby increasing the number of inactivated I(A) channels at the resting potential. I(A) currents were relatively unaffected by 1 mM CTP-cAMP or 500 nM phorbol-12, 13-dibuterate (a protein kinase C agonist) but were inhibited by 20-30% with either 1 mM CTP-cGMP or 25 microM N-(6-aminohexyl)-5-chloro-1-napthalenesulfonamide HCl (a calcium-calmodulin kinase inhibitor). In the presence of 0.5 microM KT5823, an inhibitor of protein kinase G (PKG) pathways, 1 microM capsaicin inhibited I(A) by only 26%. In summary, in CS neurons, capsaicin decreases I(A) currents through the activation of vanilloid receptors. That activation, partially through the activation of cGMP-PKG and calmodulin-dependent pathways should result in increased excitability of capsaicin-sensitive nociceptors.

Duke Scholars

Published In

J Neurophysiol

DOI

ISSN

0022-3077

Publication Date

March 2003

Volume

89

Issue

3

Start / End Page

1387 / 1401

Location

United States

Related Subject Headings

  • Trigeminal Ganglion
  • Sulfonamides
  • Receptors, Drug
  • Rats, Sprague-Dawley
  • Rats
  • Potassium Channels, Voltage-Gated
  • Potassium
  • Phorbol 12,13-Dibutyrate
  • Patch-Clamp Techniques
  • Nociceptors
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Liu, L., & Simon, S. A. (2003). Modulation of IA currents by capsaicin in rat trigeminal ganglion neurons. J Neurophysiol, 89(3), 1387–1401. https://doi.org/10.1152/jn.00210.2002
Liu, L., and S. A. Simon. “Modulation of IA currents by capsaicin in rat trigeminal ganglion neurons.J Neurophysiol 89, no. 3 (March 2003): 1387–1401. https://doi.org/10.1152/jn.00210.2002.
Liu L, Simon SA. Modulation of IA currents by capsaicin in rat trigeminal ganglion neurons. J Neurophysiol. 2003 Mar;89(3):1387–401.
Liu, L., and S. A. Simon. “Modulation of IA currents by capsaicin in rat trigeminal ganglion neurons.J Neurophysiol, vol. 89, no. 3, Mar. 2003, pp. 1387–401. Pubmed, doi:10.1152/jn.00210.2002.
Liu L, Simon SA. Modulation of IA currents by capsaicin in rat trigeminal ganglion neurons. J Neurophysiol. 2003 Mar;89(3):1387–1401.

Published In

J Neurophysiol

DOI

ISSN

0022-3077

Publication Date

March 2003

Volume

89

Issue

3

Start / End Page

1387 / 1401

Location

United States

Related Subject Headings

  • Trigeminal Ganglion
  • Sulfonamides
  • Receptors, Drug
  • Rats, Sprague-Dawley
  • Rats
  • Potassium Channels, Voltage-Gated
  • Potassium
  • Phorbol 12,13-Dibutyrate
  • Patch-Clamp Techniques
  • Nociceptors