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Acidic stimuli activates two distinct pathways in taste receptor cells from rat fungiform papillae.

Publication ,  Journal Article
Liu, L; Simon, SA
Published in: Brain Res
December 27, 2001

A sour taste sensation may be produced when acidic stimuli interact with taste receptor cells (TRCs) on the dorsal surface of the tongue. We have searched for pathways in TRCs that may be activated by acidic stimuli using RT-PCR and changes in intracellular calcium (Ca(2+)(I)) induced by acidic stimuli in rat fungiform papillae. RT-PCR revealed the presence of proton-gated subunits ASIC-beta and VR1. Ca(2+) imaging measurements of the TRCs revealed two distinct responses to acidic stimuli: Ca(2+)(i) was increased in 9% (28/308; Type I) and was decreased in 39% (121/308; Type II). Neither of these responses was affected by the removal of extracellular Ca(2+), indicating that the changes arise from the release and sequestration of Ca(2+) from intracellular stores. These responses were also not inhibited by the vanilloid receptor antagonist, capsazepine, suggesting they do not arise from the activation of vanilloid receptors. The Type I, but not the Type II response was inhibited by amiloride. Dose-response measurements for Types I and II responses yielded pH(50%) of 4.8 and 4.9, respectively. Type II responses were inhibited by pertussis toxin, suggesting G-protein involvement. TRCs that exhibit Type II responses could also be activated by quinine (which increased Ca(2+)(I)) thus suggesting a mechanism by which the addition of acid may be suppressive to other chemical stimuli.

Duke Scholars

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

December 27, 2001

Volume

923

Issue

1-2

Start / End Page

58 / 70

Location

Netherlands

Related Subject Headings

  • Virulence Factors, Bordetella
  • Taste Buds
  • Taste
  • Tachyphylaxis
  • Stimulation, Chemical
  • Sodium Channels
  • Reverse Transcriptase Polymerase Chain Reaction
  • Receptors, Drug
  • Rats, Sprague-Dawley
  • Rats
 

Citation

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Liu, L., & Simon, S. A. (2001). Acidic stimuli activates two distinct pathways in taste receptor cells from rat fungiform papillae. Brain Res, 923(1–2), 58–70. https://doi.org/10.1016/s0006-8993(01)03190-0
Liu, L., and S. A. Simon. “Acidic stimuli activates two distinct pathways in taste receptor cells from rat fungiform papillae.Brain Res 923, no. 1–2 (December 27, 2001): 58–70. https://doi.org/10.1016/s0006-8993(01)03190-0.
Liu, L., and S. A. Simon. “Acidic stimuli activates two distinct pathways in taste receptor cells from rat fungiform papillae.Brain Res, vol. 923, no. 1–2, Dec. 2001, pp. 58–70. Pubmed, doi:10.1016/s0006-8993(01)03190-0.
Journal cover image

Published In

Brain Res

DOI

ISSN

0006-8993

Publication Date

December 27, 2001

Volume

923

Issue

1-2

Start / End Page

58 / 70

Location

Netherlands

Related Subject Headings

  • Virulence Factors, Bordetella
  • Taste Buds
  • Taste
  • Tachyphylaxis
  • Stimulation, Chemical
  • Sodium Channels
  • Reverse Transcriptase Polymerase Chain Reaction
  • Receptors, Drug
  • Rats, Sprague-Dawley
  • Rats