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Residual oil fly ash and charged polymers activate epithelial cells and nociceptive sensory neurons.

Publication ,  Journal Article
Oortgiesen, M; Veronesi, B; Eichenbaum, G; Kiser, PF; Simon, SA
Published in: Am J Physiol Lung Cell Mol Physiol
April 2000

Residual oil fly ash (ROFA) is an industrial pollutant that contains metals, acids, and unknown materials complexed to a particulate core. The heterogeneous composition of ROFA hampers finding the mechanism(s) by which it and other particulate pollutants cause airway toxicity. To distinguish culpable factors contributing to the effects of ROFA, synthetic polymer microsphere (SPM) analogs were synthesized that resembled ROFA in particle size (2 and 6 microm in diameter) and zeta potential (-29 mV). BEAS-2B human bronchial epithelial cells and dorsal root ganglion neurons responded to both ROFA and charged SPMs with an increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) and the release of the proinflammatory cytokine interleukin-6, whereas neutral SPMs bound with polyethylene glycol (0-mV zeta potential) were relatively ineffective. In dorsal root ganglion neurons, the SPM-induced increases in [Ca(2+)](i) were correlated with the presence of acid- and/or capsaicin-sensitive pathways. We hypothesized that the acidic microenvironment associated with negatively charged colloids like ROFA and SPMs activate irritant receptors in airway target cells. This causes subsequent cytokine release, which mediates the pathophysiology of neurogenic airway inflammation.

Duke Scholars

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

April 2000

Volume

278

Issue

4

Start / End Page

L683 / L695

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Respiratory System
  • Polymers
  • Polyethylene Glycols
  • Particulate Matter
  • Osmolar Concentration
  • Nociceptors
  • Neurons, Afferent
  • Microspheres
  • Intracellular Membranes
 

Citation

APA
Chicago
ICMJE
MLA
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Oortgiesen, M., Veronesi, B., Eichenbaum, G., Kiser, P. F., & Simon, S. A. (2000). Residual oil fly ash and charged polymers activate epithelial cells and nociceptive sensory neurons. Am J Physiol Lung Cell Mol Physiol, 278(4), L683–L695. https://doi.org/10.1152/ajplung.2000.278.4.L683
Oortgiesen, M., B. Veronesi, G. Eichenbaum, P. F. Kiser, and S. A. Simon. “Residual oil fly ash and charged polymers activate epithelial cells and nociceptive sensory neurons.Am J Physiol Lung Cell Mol Physiol 278, no. 4 (April 2000): L683–95. https://doi.org/10.1152/ajplung.2000.278.4.L683.
Oortgiesen M, Veronesi B, Eichenbaum G, Kiser PF, Simon SA. Residual oil fly ash and charged polymers activate epithelial cells and nociceptive sensory neurons. Am J Physiol Lung Cell Mol Physiol. 2000 Apr;278(4):L683–95.
Oortgiesen, M., et al. “Residual oil fly ash and charged polymers activate epithelial cells and nociceptive sensory neurons.Am J Physiol Lung Cell Mol Physiol, vol. 278, no. 4, Apr. 2000, pp. L683–95. Pubmed, doi:10.1152/ajplung.2000.278.4.L683.
Oortgiesen M, Veronesi B, Eichenbaum G, Kiser PF, Simon SA. Residual oil fly ash and charged polymers activate epithelial cells and nociceptive sensory neurons. Am J Physiol Lung Cell Mol Physiol. 2000 Apr;278(4):L683–L695.

Published In

Am J Physiol Lung Cell Mol Physiol

DOI

ISSN

1040-0605

Publication Date

April 2000

Volume

278

Issue

4

Start / End Page

L683 / L695

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Respiratory System
  • Polymers
  • Polyethylene Glycols
  • Particulate Matter
  • Osmolar Concentration
  • Nociceptors
  • Neurons, Afferent
  • Microspheres
  • Intracellular Membranes