Primary structure and transcriptional regulation of GAP-43, a protein associated with nerve growth.

Published

Journal Article

Nerve regeneration and developmental outgrowth of axons are both correlated with increased synthesis of an axonal membrane protein designated GAP-43. Phosphorylation of an apparently identical protein, present at lower abundance in adult brains, has been correlated with long-term potentiation, a form of synaptic plasticity. We have now isolated a cDNA clone encoding GAP-43 from neonatal rat brain. The amino acid sequence is extremely hydrophilic, with no potential membrane-spanning domains and no sites for N-linked glycosylation, but with a short hydrophobic segment at the protein's amino terminus, consistent with a model in which GAP-43 extends from the cytoplasmic surface of growth cone and synaptic plasma membranes. Among several tissues and cells examined, GAP-43 mRNA is expressed only in neurons. Developmental and regeneration-associated changes in GAP-43 synthesis appear to be mediated largely at the level of transcription of a single gene.

Full Text

Duke Authors

Cited Authors

  • Basi, GS; Jacobson, RD; Virág, I; Schilling, J; Skene, JH

Published Date

  • June 1, 1987

Published In

Volume / Issue

  • 49 / 6

Start / End Page

  • 785 - 791

PubMed ID

  • 3581170

Pubmed Central ID

  • 3581170

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/0092-8674(87)90616-7

Language

  • eng