Genome-wide high-resolution mapping of exosome substrates reveals hidden features in the Arabidopsis transcriptome.

Published

Journal Article

The exosome complex plays a central and essential role in RNA metabolism. However, comprehensive studies of exosome substrates and functional analyses of its subunits are lacking. Here, we demonstrate that as opposed to yeast and metazoans the plant exosome core possesses an unanticipated functional plasticity and present a genome-wide atlas of Arabidopsis exosome targets. Additionally, our study provides evidence for widespread polyadenylation- and exosome-mediated RNA quality control in plants, reveals unexpected aspects of stable structural RNA metabolism, and uncovers numerous novel exosome substrates. These include a select subset of mRNAs, miRNA processing intermediates, and hundreds of noncoding RNAs, the vast majority of which have not been previously described and belong to a layer of the transcriptome that can only be visualized upon inhibition of exosome activity. These first genome-wide maps of exosome substrates will aid in illuminating new fundamental components and regulatory mechanisms of eukaryotic transcriptomes.

Full Text

Duke Authors

Cited Authors

  • Chekanova, JA; Gregory, BD; Reverdatto, SV; Chen, H; Kumar, R; Hooker, T; Yazaki, J; Li, P; Skiba, N; Peng, Q; Alonso, J; Brukhin, V; Grossniklaus, U; Ecker, JR; Belostotsky, DA

Published Date

  • December 28, 2007

Published In

Volume / Issue

  • 131 / 7

Start / End Page

  • 1340 - 1353

PubMed ID

  • 18160042

Pubmed Central ID

  • 18160042

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/j.cell.2007.10.056

Language

  • eng

Conference Location

  • United States