Skip to main content

[Synthesis of human proinsulin in Escherichia coli cells].

Publication ,  Journal Article
Efimov, VA; Aleksiuk, IV; Buriakova, AA; Pashkova, IN; Skiba, NP; Chakhmakhcheva, OG
Published in: Bioorg Khim
August 1989

Expression of the synthetic gene for human proinsulin in E. coli has been investigated. The proinsulin gene has been expressed directly under the control of a synthetic promoter of phage fd DNA and a promoter of tryptophan operon, or using fusions with fragments of some bacterial proteins. These fusions gave insoluble polypeptide products amounting to 20-30% of total cellular protein. The scheme for isolating proinsulin from bacterial cells was developed. Proinsulin was cleaved from leader polypeptides by treatment with cyanogen bromide and converted into human insulin.

Duke Scholars

Published In

Bioorg Khim

ISSN

0132-3423

Publication Date

August 1989

Volume

15

Issue

8

Start / End Page

1078 / 1090

Location

Russia (Federation)

Related Subject Headings

  • Recombinant Proteins
  • Radioimmunoassay
  • Promoter Regions, Genetic
  • Proinsulin
  • Plasmids
  • Humans
  • Gene Expression Regulation, Viral
  • Gene Expression Regulation, Bacterial
  • Escherichia coli
  • Electrophoresis, Polyacrylamide Gel
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Efimov, V. A., Aleksiuk, I. V., Buriakova, A. A., Pashkova, I. N., Skiba, N. P., & Chakhmakhcheva, O. G. (1989). [Synthesis of human proinsulin in Escherichia coli cells]. Bioorg Khim, 15(8), 1078–1090.
Efimov, V. A., I. V. Aleksiuk, A. A. Buriakova, I. N. Pashkova, N. P. Skiba, and O. G. Chakhmakhcheva. “[Synthesis of human proinsulin in Escherichia coli cells].Bioorg Khim 15, no. 8 (August 1989): 1078–90.
Efimov VA, Aleksiuk IV, Buriakova AA, Pashkova IN, Skiba NP, Chakhmakhcheva OG. [Synthesis of human proinsulin in Escherichia coli cells]. Bioorg Khim. 1989 Aug;15(8):1078–90.
Efimov, V. A., et al. “[Synthesis of human proinsulin in Escherichia coli cells].Bioorg Khim, vol. 15, no. 8, Aug. 1989, pp. 1078–90.
Efimov VA, Aleksiuk IV, Buriakova AA, Pashkova IN, Skiba NP, Chakhmakhcheva OG. [Synthesis of human proinsulin in Escherichia coli cells]. Bioorg Khim. 1989 Aug;15(8):1078–1090.

Published In

Bioorg Khim

ISSN

0132-3423

Publication Date

August 1989

Volume

15

Issue

8

Start / End Page

1078 / 1090

Location

Russia (Federation)

Related Subject Headings

  • Recombinant Proteins
  • Radioimmunoassay
  • Promoter Regions, Genetic
  • Proinsulin
  • Plasmids
  • Humans
  • Gene Expression Regulation, Viral
  • Gene Expression Regulation, Bacterial
  • Escherichia coli
  • Electrophoresis, Polyacrylamide Gel