[Synthesis of human proinsulin in Escherichia coli cells].
Expression of the synthetic gene for human proinsulin in E. coli has been investigated. The proinsulin gene has been expressed directly under the control of a synthetic promoter of phage fd DNA and a promoter of tryptophan operon, or using fusions with fragments of some bacterial proteins. These fusions gave insoluble polypeptide products amounting to 20-30% of total cellular protein. The scheme for isolating proinsulin from bacterial cells was developed. Proinsulin was cleaved from leader polypeptides by treatment with cyanogen bromide and converted into human insulin.
Efimov, VA; Aleksiuk, IV; Buriakova, AA; Pashkova, IN; Skiba, NP; Chakhmakhcheva, OG
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