Coordinate reduction of rat pancreatic islet glucokinase and proinsulin mRNA by exercise training.


Journal Article

Exercise training results not only in enhanced insulin sensitivity but also in a reduction in insulin secretion. In this study, we examined the effects of exercise training on the expression of genes potentially related to insulin synthesis and glucose-stimulated insulin release by measuring pancreatic islet proinsulin, glucose-transporter (GLUT2), and glucokinase mRNAs. Female Wistar rats were subjected to 100 min of running at 25 m.min-1 up a 15% incline for 90 min/day for 6 days/wk for 3 wk. Pancreatic mRNA was evaluated by Northern- and dot-blot analysis with [32P]cRNA probes. We found no change in the pancreatic content of GLUT2 mRNA but found marked decreases in the content of proinsulin mRNA (78%, P less than 0.005) and glucokinase mRNA (65%, P less than 0.001). These results suggest that exercise modulates both islet glucose metabolism and insulin synthesis at the level of gene expression. Furthermore, there was a significant correlation between the decreases in glucokinase and proinsulin mRNA concentrations (r = 0.95, P less than 0.001), suggesting that expression of these genes is regulated in parallel.

Full Text

Duke Authors

Cited Authors

  • Koranyi, LI; Bourey, RE; Slentz, CA; Holloszy, JO; Permutt, MA

Published Date

  • March 1, 1991

Published In

Volume / Issue

  • 40 / 3

Start / End Page

  • 401 - 404

PubMed ID

  • 1705526

Pubmed Central ID

  • 1705526

International Standard Serial Number (ISSN)

  • 0012-1797

Digital Object Identifier (DOI)

  • 10.2337/diab.40.3.401


  • eng

Conference Location

  • United States