Phospholipase C-beta 2 interacts with mitogen-activated protein kinase kinase 3.

Published

Journal Article

Phospholipase C (PLC)-beta enzymes (isoenzymes beta 1-beta 4) are activated by G protein subunits, leading to the generation of intracellular messengers which mobilize calcium and activate protein kinase C. It has recently been recognized that these enzymes interact with and are regulated by proteins other than G proteins. Using the yeast two-hybrid technique to screen a leukocyte library we identified mitogen-activated protein kinase kinase 3 (MKK3) as a partner of PLC-beta 2. The interaction was confirmed by co-immunoprecipitation assays which indicated that MKK3 interacts with PLC-beta 2, but not with other PLC-betas. PLC-beta 2 interacted weakly with MKK6, which is related to MKK3, but not with the other MKK3 tested. The region of PLC-beta 2 involved in the interaction with MKK3 was mapped to the C-terminus of PLC-beta 2. p38MAPK also co-immunoprecipitated with PLC-beta 2. The data suggest that PLC-beta 2 serves an unappreciated role assembling components of the p38MAPK signaling module.

Full Text

Duke Authors

Cited Authors

  • Barr, AJ; Marjoram, R; Xu, J; Snyderman, R

Published Date

  • April 26, 2002

Published In

Volume / Issue

  • 293 / 1

Start / End Page

  • 647 - 652

PubMed ID

  • 12054652

Pubmed Central ID

  • 12054652

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/S0006-291X(02)00259-0

Language

  • eng

Conference Location

  • United States