Functional high efficiency expression of cloned leukocyte chemoattractant receptor cDNAs.

Published

Journal Article

Human kidney 293 TSA cells were transfected by a calcium phosphate method with human formylpeptide and C5a receptor cDNAs with high efficiency. Formylpeptide receptor positive transfectants expressed a total of 968,000 +/- 34,000 receptors per cell with two affinity states (Kds of ca. 0.43 nM and 39 nM), which in the presence of 100 microM GTP gamma S decreased by ca. 4-fold the number of high-affinity sites. The ligand binding pharmacology of cloned and expressed formylpeptide receptors were indistinguishable from endogenous receptors on human neutrophils. Expressed formylpeptide and C5a receptors were functionally active in mobilizing intracellular calcium via a pertussis toxin sensitive mechanism with an ED50 for formylpeptide of ca. 0.5-1.0 nM. This expression system, in which receptor expression can be monitored by flow cytometric methods and in which intracellular calcium responses are measurable, unlike in the more popular COS-7 cell expression system, will provide a useful basis for the analysis of chemoattractant receptor structure-function relationships.

Full Text

Duke Authors

Cited Authors

  • Didsbury, JR; Uhing, RJ; Tomhave, E; Gerard, C; Gerard, N; Snyderman, R

Published Date

  • February 10, 1992

Published In

Volume / Issue

  • 297 / 3

Start / End Page

  • 275 - 279

PubMed ID

  • 1544408

Pubmed Central ID

  • 1544408

International Standard Serial Number (ISSN)

  • 0014-5793

Digital Object Identifier (DOI)

  • 10.1016/0014-5793(92)80555-u

Language

  • eng

Conference Location

  • England