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Leukocyte chemoattraction by 1,2-diacylglycerol.

Publication ,  Journal Article
Wright, TM; Hoffman, RD; Nishijima, J; Jakoi, L; Snyderman, R; Shin, HS
Published in: Proc Natl Acad Sci U S A
March 1988

Previous reports have demonstrated the hydrolysis of inositol phospholipids in polymorphonuclear leukocytes (PMN) in response to chemoattractants and in lymphocytes in response to the mitogen phytohemagglutinin. We investigated the role of 1,2-diacylglycerol, one of the products of receptor-linked phosphatidylinositol hydrolysis, in mediating the migratory response of leukocytes. In an under-agarose migration system, we found 1,2-dioctanoylglycerol to be a strong chemoattractant for human PMN, 6C3HED (a mouse thymic lymphoma), and Jurkat (a human T-cell leukemia). By using a modified Boyden chamber assay, the migratory response of PMN to 1,2-dioctanoylglycerol was determined to be primarily chemotactic. Analysis of structural analogs indicated that both the position and number of acyl chains are important in determining chemoattractant activity. These studies demonstrate that exogenous 1,2-diacylglycerol can stimulate the directed migration of leukocytes. They further suggest that the formation of 1,2-diacylglycerol following receptor-mediated stimulation may represent a common step in the migratory responses of myeloid and lymphoid cells.

Duke Scholars

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

March 1988

Volume

85

Issue

6

Start / End Page

1869 / 1873

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Structure-Activity Relationship
  • Protein Kinase C
  • Phosphatidylinositols
  • Neutrophils
  • Mice
  • Leukemia, Experimental
  • Humans
  • Glycerides
  • Diglycerides
 

Citation

APA
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ICMJE
MLA
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Wright, T. M., Hoffman, R. D., Nishijima, J., Jakoi, L., Snyderman, R., & Shin, H. S. (1988). Leukocyte chemoattraction by 1,2-diacylglycerol. Proc Natl Acad Sci U S A, 85(6), 1869–1873. https://doi.org/10.1073/pnas.85.6.1869
Wright, T. M., R. D. Hoffman, J. Nishijima, L. Jakoi, R. Snyderman, and H. S. Shin. “Leukocyte chemoattraction by 1,2-diacylglycerol.Proc Natl Acad Sci U S A 85, no. 6 (March 1988): 1869–73. https://doi.org/10.1073/pnas.85.6.1869.
Wright TM, Hoffman RD, Nishijima J, Jakoi L, Snyderman R, Shin HS. Leukocyte chemoattraction by 1,2-diacylglycerol. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1869–73.
Wright, T. M., et al. “Leukocyte chemoattraction by 1,2-diacylglycerol.Proc Natl Acad Sci U S A, vol. 85, no. 6, Mar. 1988, pp. 1869–73. Pubmed, doi:10.1073/pnas.85.6.1869.
Wright TM, Hoffman RD, Nishijima J, Jakoi L, Snyderman R, Shin HS. Leukocyte chemoattraction by 1,2-diacylglycerol. Proc Natl Acad Sci U S A. 1988 Mar;85(6):1869–1873.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

ISSN

0027-8424

Publication Date

March 1988

Volume

85

Issue

6

Start / End Page

1869 / 1873

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Structure-Activity Relationship
  • Protein Kinase C
  • Phosphatidylinositols
  • Neutrophils
  • Mice
  • Leukemia, Experimental
  • Humans
  • Glycerides
  • Diglycerides