Leukocyte chemoattraction by 1,2-diacylglycerol.

Journal Article (Journal Article)

Previous reports have demonstrated the hydrolysis of inositol phospholipids in polymorphonuclear leukocytes (PMN) in response to chemoattractants and in lymphocytes in response to the mitogen phytohemagglutinin. We investigated the role of 1,2-diacylglycerol, one of the products of receptor-linked phosphatidylinositol hydrolysis, in mediating the migratory response of leukocytes. In an under-agarose migration system, we found 1,2-dioctanoylglycerol to be a strong chemoattractant for human PMN, 6C3HED (a mouse thymic lymphoma), and Jurkat (a human T-cell leukemia). By using a modified Boyden chamber assay, the migratory response of PMN to 1,2-dioctanoylglycerol was determined to be primarily chemotactic. Analysis of structural analogs indicated that both the position and number of acyl chains are important in determining chemoattractant activity. These studies demonstrate that exogenous 1,2-diacylglycerol can stimulate the directed migration of leukocytes. They further suggest that the formation of 1,2-diacylglycerol following receptor-mediated stimulation may represent a common step in the migratory responses of myeloid and lymphoid cells.

Full Text

Duke Authors

Cited Authors

  • Wright, TM; Hoffman, RD; Nishijima, J; Jakoi, L; Snyderman, R; Shin, HS

Published Date

  • March 1988

Published In

Volume / Issue

  • 85 / 6

Start / End Page

  • 1869 - 1873

PubMed ID

  • 3162313

Pubmed Central ID

  • PMC279882

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.85.6.1869


  • eng

Conference Location

  • United States