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Altered cell-averaged microviscosity of murine peritoneal macrophages undergoing activation in vivo or in vitro.

Publication ,  Journal Article
Somers, SD; Yuli, I; Snyderman, R; Adams, DO
Published in: Cell Immunol
February 1987

The cell-averaged microviscosity of intact murine peritoneal mononuclear phagocytes in various stages of activation was assessed by quantifying fluorescent depolarization of 1,6-diphenyl-1,3,5-hexatriene. Macrophages activated in vivo with Mycobacterium bovis, strain BCG, were significantly more fluid than resident peritoneal macrophages, responsive macrophages elicited with thioglycollate broth, proteose peptone broth, or fetal bovine serum, or primed macrophages elicited with pyran copolymer, MVE-2. Specifically, the cell-averaged microviscosity decreased from a mean of 3.47 +/- .07 eta 25 degrees C (poise) (range of 3.32 to 3.67 p) to 2.62 eta 25 degrees C. Exposure of responsive macrophages in vitro to bacterial endotoxin plus hybridoma supernatants containing macrophage-activating factor or purified recombinant interferon gamma resulted in decreased microviscosity; the largest effect was seen after 24 hr. Macrophages primed in vivo with MVE-2 and treated in vitro with endotoxin also developed decreased microviscosity. Similar changes in microviscosity were observed in a plasma membrane-enriched fraction isolated from macrophages activated in vitro with interferon gamma and endotoxin, thus suggesting that the cell-averaged measurements reflected changes in membrane viscosity. The optimum concentration of MAF-inducing decreased overall microviscosity was identical to that for inducing tumoricidal capacity. Taken together, the data indicate activation of lytic capacity in murine macrophages is closely associated with decreased cell-averaged microviscosity and that this change reflects, at least in part, decreased microviscosity of the plasma membrane of these cells.

Duke Scholars

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

February 1987

Volume

104

Issue

2

Start / End Page

232 / 244

Location

Netherlands

Related Subject Headings

  • Viscosity
  • Mice
  • Membrane Fluidity
  • Macrophages
  • Macrophage-Activating Factors
  • Macrophage Activation
  • Lymphokines
  • Inflammation
  • Immunology
  • Endotoxins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Somers, S. D., Yuli, I., Snyderman, R., & Adams, D. O. (1987). Altered cell-averaged microviscosity of murine peritoneal macrophages undergoing activation in vivo or in vitro. Cell Immunol, 104(2), 232–244. https://doi.org/10.1016/0008-8749(87)90026-8
Somers, S. D., I. Yuli, R. Snyderman, and D. O. Adams. “Altered cell-averaged microviscosity of murine peritoneal macrophages undergoing activation in vivo or in vitro.Cell Immunol 104, no. 2 (February 1987): 232–44. https://doi.org/10.1016/0008-8749(87)90026-8.
Somers SD, Yuli I, Snyderman R, Adams DO. Altered cell-averaged microviscosity of murine peritoneal macrophages undergoing activation in vivo or in vitro. Cell Immunol. 1987 Feb;104(2):232–44.
Somers, S. D., et al. “Altered cell-averaged microviscosity of murine peritoneal macrophages undergoing activation in vivo or in vitro.Cell Immunol, vol. 104, no. 2, Feb. 1987, pp. 232–44. Pubmed, doi:10.1016/0008-8749(87)90026-8.
Somers SD, Yuli I, Snyderman R, Adams DO. Altered cell-averaged microviscosity of murine peritoneal macrophages undergoing activation in vivo or in vitro. Cell Immunol. 1987 Feb;104(2):232–244.
Journal cover image

Published In

Cell Immunol

DOI

ISSN

0008-8749

Publication Date

February 1987

Volume

104

Issue

2

Start / End Page

232 / 244

Location

Netherlands

Related Subject Headings

  • Viscosity
  • Mice
  • Membrane Fluidity
  • Macrophages
  • Macrophage-Activating Factors
  • Macrophage Activation
  • Lymphokines
  • Inflammation
  • Immunology
  • Endotoxins