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Human Gi protein alpha-subunit: deduction of amino acid structure from a cloned cDNA.

Publication ,  Journal Article
Didsbury, JR; Ho, YS; Snyderman, R
Published in: FEBS Lett
January 26, 1987

The amino acid sequence of the alpha-subunit of Gi, the human adenylate cyclase inhibiting GTP-binding protein, has been deduced from the nucleotide sequence of a DNA clone complementary to Gi alpha mRNA from differentiated U937 cells. The cDNA encodes a polypeptide of 355 amino acids (Mr 40456). The amino acid sequence homology between human Gi alpha and rat, murine, and bovine Gi alpha is 98.6, 97.7 and 87.9% respectively. Differentiation of the U937 cells from monoblasts to monocyte-like cells resulted in a 3-fold increase in Gi alpha mRNA as well as a 3.6-fold increase in the 41 kDa pertussis toxin substrate presumed to be Gi alpha. Thus, increased levels of this G-protein are associated with monocyte differentiation and appear to be regulated transcriptionally.

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Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

January 26, 1987

Volume

211

Issue

2

Start / End Page

160 / 164

Location

England

Related Subject Headings

  • Humans
  • GTP-Binding Proteins
  • DNA, Circular
  • Cloning, Molecular
  • Biochemistry & Molecular Biology
  • Base Sequence
  • Amino Acid Sequence
  • Adenylyl Cyclase Inhibitors
  • 3101 Biochemistry and cell biology
  • 0603 Evolutionary Biology
 

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Didsbury, J. R., Ho, Y. S., & Snyderman, R. (1987). Human Gi protein alpha-subunit: deduction of amino acid structure from a cloned cDNA. FEBS Lett, 211(2), 160–164. https://doi.org/10.1016/0014-5793(87)81428-x
Didsbury, J. R., Y. S. Ho, and R. Snyderman. “Human Gi protein alpha-subunit: deduction of amino acid structure from a cloned cDNA.FEBS Lett 211, no. 2 (January 26, 1987): 160–64. https://doi.org/10.1016/0014-5793(87)81428-x.
Didsbury JR, Ho YS, Snyderman R. Human Gi protein alpha-subunit: deduction of amino acid structure from a cloned cDNA. FEBS Lett. 1987 Jan 26;211(2):160–4.
Didsbury, J. R., et al. “Human Gi protein alpha-subunit: deduction of amino acid structure from a cloned cDNA.FEBS Lett, vol. 211, no. 2, Jan. 1987, pp. 160–64. Pubmed, doi:10.1016/0014-5793(87)81428-x.
Didsbury JR, Ho YS, Snyderman R. Human Gi protein alpha-subunit: deduction of amino acid structure from a cloned cDNA. FEBS Lett. 1987 Jan 26;211(2):160–164.
Journal cover image

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

January 26, 1987

Volume

211

Issue

2

Start / End Page

160 / 164

Location

England

Related Subject Headings

  • Humans
  • GTP-Binding Proteins
  • DNA, Circular
  • Cloning, Molecular
  • Biochemistry & Molecular Biology
  • Base Sequence
  • Amino Acid Sequence
  • Adenylyl Cyclase Inhibitors
  • 3101 Biochemistry and cell biology
  • 0603 Evolutionary Biology