Quantification of mouse macrophage chemotaxis in vitro: role of C5 for the production of chemotactic activity.

Journal Article

Delineation of the mechanisms of macrophage accumulation at local tissue sites will further our understanding of immunologically mediated host resistance to infectious and neoplastic diseases. Since mice are frequently used for the study of immune function, we developed a method for the quantification of mouse macrophage chemotaxis in vitro. By this method it was found that the fifth component of complement is necessary for the production of chemotactic activity in mouse serum by inflammatory agents such as endotoxin or aggregated gamma globulin. The majority of macrophage chemotactic activity produced by these agents in mouse serum can be attributed to a low-molecular-weight (ca. 15,000) chemotactic factor. The data suggest that this factor is the biologically active cleavage product of the fifth component of complement, C5a.

Full Text

Duke Authors

Cited Authors

  • Snyderman, R; Pike, MC; McCarley, D; Lang, L

Published Date

  • March 1, 1975

Published In

Volume / Issue

  • 11 / 3

Start / End Page

  • 488 - 492

PubMed ID

  • 46841

International Standard Serial Number (ISSN)

  • 0019-9567

Language

  • eng

Conference Location

  • United States