Interaction of complex polysaccharides with the complement system: effect of calcium depletion on terminal component consumption.
Journal Article
Complex polysaccharides and lipopolysaccharides can activate the terminal components of complement by either the classical (antibody, C1, C4, and C2) or alternative complement pathways, but the relative importance of either pathway for terminal component consumption in normal serum is poorly understood. Since classical complement pathway function requires both calcium and magnesium ions, whereas the alternative pathway requires only magnesium ions, selective chelation of calcium ions in serum can be used to block the classical complement pathway while leaving the alternative pathway intact. In these studies, ethyleneglycol-bis-(beta-aminoethyl ether)N, N-tetraacetic acid, a potent chelator or calcium, was used to block the classical complement pathway in normal guinea pig serum. Consumption of the terminal complement components by endotoxin, inulin, and zymosan in such serum was strikingly depressed when compared to serum containing an intact classical complement pathway. These studies demonstrate that in normal serum, both the classical and alternative complement pathways participate in the consumption of the terminal complement components by complex polysaccharides and lipopolysaccharides.
Full Text
Duke Authors
Cited Authors
- Snyderman, R; Pike, MC
Published Date
- February 1, 1975
Published In
Volume / Issue
- 11 / 2
Start / End Page
- 273 - 279
PubMed ID
- 46215
Pubmed Central ID
- 46215
International Standard Serial Number (ISSN)
- 0019-9567
Digital Object Identifier (DOI)
- 10.1128/IAI.11.2.273-279.1975
Language
- eng
Conference Location
- United States