Interstitial volume changes during spreading depression (SD) and SD-like hypoxic depolarization in hippocampal tissue slices.


Journal Article

1. Relative interstitial volume (ISV) was estimated from the concentration changes of iontophoretically administered tetramethyl- and tetraethylammonium (TMA+ and TEA+). Spreading depression (SD) was provoked by high K+, and hypoxic SD-like depolarization (HSD) was induced by withdrawing oxygen. 2. Probe ion concentrations increased dramatically and about equally during SD and HSD, except that in a few hypoxic trials signals became transiently smaller than control. Interstitial volume appeared to decrease on the average by approximately 70%. 3. The ISV that remains patent in CA1 region at the height of SD is < 4% of total tissue volume. Probe ions may occasionally have passed through cell membranes for a short time during hypoxic SD.

Full Text

Duke Authors

Cited Authors

  • Jing, J; Aitken, PG; Somjen, GG

Published Date

  • June 1, 1994

Published In

Volume / Issue

  • 71 / 6

Start / End Page

  • 2548 - 2551

PubMed ID

  • 7523614

Pubmed Central ID

  • 7523614

International Standard Serial Number (ISSN)

  • 0022-3077

Digital Object Identifier (DOI)

  • 10.1152/jn.1994.71.6.2548


  • eng

Conference Location

  • United States