A Haemophilus influenzae IgA protease-like protein promotes intimate interaction with human epithelial cells.


Journal Article

Haemophilus influenzae represents a common cause of human disease and an important source of morbidity and mortality. Disease caused by this organism begins with colonization of the upper respiratory tract. Several studies indicate that H. influenzae is capable of binding to and entering cultured human cells, properties which are potentially of relevance to the process of colonization. In the present study, we isolated an H. influenzae gene designated hap, which is associated with the capacity for in vitro attachment and entry. Analysis of the derived amino acid sequence of hap demonstrated significant homology with the serine-type IgA1 proteases expressed by H. influenzae and Neisseria gonorrhoeae. It is notable that the hap product shares the catalytic domain of the IgA1 proteases and appears to be processed and secreted in an analogous manner. We speculate that the hap gene product is an important determinant of colonization, perhaps enabling the organism to evade the local immune response and thereby persist within the respiratory tract.

Full Text

Cited Authors

  • St Geme, JW; de la Morena, ML; Falkow, S

Published Date

  • October 1994

Published In

Volume / Issue

  • 14 / 2

Start / End Page

  • 217 - 233

PubMed ID

  • 7830568

Pubmed Central ID

  • 7830568

Electronic International Standard Serial Number (EISSN)

  • 1365-2958

International Standard Serial Number (ISSN)

  • 0950-382X

Digital Object Identifier (DOI)

  • 10.1111/j.1365-2958.1994.tb01283.x


  • eng