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Stimulation of aquaporin-mediated fluid transport by cyclic GMP in human retinal pigment epithelium in vitro.

Publication ,  Journal Article
Baetz, NW; Stamer, WD; Yool, AJ
Published in: Invest Ophthalmol Vis Sci
April 24, 2012

PURPOSE: The retinal pigment epithelium (RPE) expresses aquaporin-1 (AQP1) and components of the natriuretic peptide signaling pathway. We hypothesized that stimulation of the natriuretic signaling pathway in RPE with atrial natriuretic peptide (ANP) and with membrane-permeable analogs of cGMP would induce a net apical-to-basal transport of fluid. METHODS: The hypothesis was tested using human RPE cultures that retain properties seen in vivo. Confluent monolayers were treated with ANP or membrane-permeable cGMP analogs in the presence of anantin, H-8, and an AQP1 inhibitor, AqB013. Fluid movement from the apical to basal chambers was measured by weight and used to calculate net fluid transport. RESULTS: Our results demonstrated a 40% increase in net apical-to-basal fluid transport by ANP (5 μM) that was inhibited completely by the ANP receptor antagonist anantin and a 60% increase in net apical-to-basal fluid transport in response to the extracellularly applied membrane-permeable cGMP analog pCPT-cGMP (50 μM), which was not affected by the protein kinase G inhibitor H-8. The aquaporin antagonist AqB013 (20 μM) inhibited the cGMP-stimulated RPE fluid flux. CONCLUSIONS: The effect of cGMP is consistent with an enhancement of the net fluid flux in RPE mediated by AQP1 channels. Pharmacologic activation of cGMP signaling and concomitant stimulation of fluid uptake from the subretinal space could offer insights into a new approach to treating or reducing the risk of retinal detachment.

Duke Scholars

Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

April 24, 2012

Volume

53

Issue

4

Start / End Page

2127 / 2132

Location

United States

Related Subject Headings

  • Xenopus laevis
  • Water
  • Retinal Pigment Epithelium
  • Peptides, Cyclic
  • Ophthalmology & Optometry
  • Isoquinolines
  • Humans
  • Dose-Response Relationship, Drug
  • Cyclic GMP-Dependent Protein Kinases
  • Cyclic GMP
 

Citation

APA
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ICMJE
MLA
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Baetz, N. W., Stamer, W. D., & Yool, A. J. (2012). Stimulation of aquaporin-mediated fluid transport by cyclic GMP in human retinal pigment epithelium in vitro. Invest Ophthalmol Vis Sci, 53(4), 2127–2132. https://doi.org/10.1167/iovs.11-8471
Baetz, Nicholas W., W Daniel Stamer, and Andrea J. Yool. “Stimulation of aquaporin-mediated fluid transport by cyclic GMP in human retinal pigment epithelium in vitro.Invest Ophthalmol Vis Sci 53, no. 4 (April 24, 2012): 2127–32. https://doi.org/10.1167/iovs.11-8471.
Baetz NW, Stamer WD, Yool AJ. Stimulation of aquaporin-mediated fluid transport by cyclic GMP in human retinal pigment epithelium in vitro. Invest Ophthalmol Vis Sci. 2012 Apr 24;53(4):2127–32.
Baetz, Nicholas W., et al. “Stimulation of aquaporin-mediated fluid transport by cyclic GMP in human retinal pigment epithelium in vitro.Invest Ophthalmol Vis Sci, vol. 53, no. 4, Apr. 2012, pp. 2127–32. Pubmed, doi:10.1167/iovs.11-8471.
Baetz NW, Stamer WD, Yool AJ. Stimulation of aquaporin-mediated fluid transport by cyclic GMP in human retinal pigment epithelium in vitro. Invest Ophthalmol Vis Sci. 2012 Apr 24;53(4):2127–2132.

Published In

Invest Ophthalmol Vis Sci

DOI

EISSN

1552-5783

Publication Date

April 24, 2012

Volume

53

Issue

4

Start / End Page

2127 / 2132

Location

United States

Related Subject Headings

  • Xenopus laevis
  • Water
  • Retinal Pigment Epithelium
  • Peptides, Cyclic
  • Ophthalmology & Optometry
  • Isoquinolines
  • Humans
  • Dose-Response Relationship, Drug
  • Cyclic GMP-Dependent Protein Kinases
  • Cyclic GMP