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Structural basement membrane components and corresponding integrins in Schlemm's canal endothelia.

Publication ,  Journal Article
VanderWyst, SS; Perkumas, KM; Read, AT; Overby, DR; Stamer, WD
Published in: Mol Vis
January 19, 2011

PURPOSE: The conventional outflow pathway provides the primary source of resistance to aqueous humor drainage, regulating intraocular pressure. Despite large pressure gradients across the inner wall of Schlemm's canal (SC), cells remain attached to their basement membrane. The goal of this study was to examine integrin-extracellular matrix binding partners of the inner wall basement membrane that facilitate attachment. METHODS: Human outflow tissues and cultured cells were analyzed by immunofluorescence and western blotting, respectively. Radial sections of human donor eyes or en face preparations of human SC inner wall were probed with antibodies that specifically recognize collagens (Type I, III, and IV), laminins (LM-332 and LM-511) and laminin-specific integrin subunits, α3, α6, β1, and β4, typical of vascular endothelia. RESULTS: Immunofluorescence studies showed collagens Type I and IV in the SC basement membrane but not collagen III. As expected with mature vascular endothelia, SC cells in situ expressed LM-511 but not LM-332. Significantly, the integrin α6 subunit was expressed uniquely by SC. En face labeling of the inner wall displayed integrin α6 colocalizing with LM α5 at the cell periphery. Western blots of cultured human SC endothelial cell monolayers confirmed expression of Type I collagen, collagen IV, LM-511, and the α6 integrin subunit. Interestingly, LM-332 was present in cultured SC cells up to 60 days post-confluence. CONCLUSIONS: Even though cells of the inner wall endure pressure gradients in the basal to apical direction, opposite of other endothelia, human SC cells express basement membrane proteins and their cognate integrins typical of vascular endothelia.

Duke Scholars

Published In

Mol Vis

EISSN

1090-0535

Publication Date

January 19, 2011

Volume

17

Start / End Page

199 / 209

Location

United States

Related Subject Headings

  • Ophthalmology & Optometry
  • Middle Aged
  • Microscopy, Fluorescence
  • Microscopy, Confocal
  • Intraocular Pressure
  • Integrins
  • Integrin alpha6
  • Infant
  • Humans
  • Gene Expression Regulation
 

Citation

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VanderWyst, S. S., Perkumas, K. M., Read, A. T., Overby, D. R., & Stamer, W. D. (2011). Structural basement membrane components and corresponding integrins in Schlemm's canal endothelia. Mol Vis, 17, 199–209.
VanderWyst, Saumya S., Kristin M. Perkumas, A Thomas Read, Darryl R. Overby, and W Daniel Stamer. “Structural basement membrane components and corresponding integrins in Schlemm's canal endothelia.Mol Vis 17 (January 19, 2011): 199–209.
VanderWyst SS, Perkumas KM, Read AT, Overby DR, Stamer WD. Structural basement membrane components and corresponding integrins in Schlemm's canal endothelia. Mol Vis. 2011 Jan 19;17:199–209.
VanderWyst, Saumya S., et al. “Structural basement membrane components and corresponding integrins in Schlemm's canal endothelia.Mol Vis, vol. 17, Jan. 2011, pp. 199–209.
VanderWyst SS, Perkumas KM, Read AT, Overby DR, Stamer WD. Structural basement membrane components and corresponding integrins in Schlemm's canal endothelia. Mol Vis. 2011 Jan 19;17:199–209.

Published In

Mol Vis

EISSN

1090-0535

Publication Date

January 19, 2011

Volume

17

Start / End Page

199 / 209

Location

United States

Related Subject Headings

  • Ophthalmology & Optometry
  • Middle Aged
  • Microscopy, Fluorescence
  • Microscopy, Confocal
  • Intraocular Pressure
  • Integrins
  • Integrin alpha6
  • Infant
  • Humans
  • Gene Expression Regulation