Alpha-2 adrenergic receptors stimulate actin organization in developing fetal rat cardiac myocytes.

Published

Journal Article

Expression of alpha(2)-adrenergic receptors (alpha(2)-AR) is very high in fetal rat heart although numbers decline with increasing gestational age. The current experiments were designed to identify the subtypes of alpha(2)-AR expressed and the function of these receptors in fetal cardiac myocytes. Expression of alpha(2)A and alpha(2)C, but not alpha(2)B, was confirmed in the myocyte population by indirect immunofluorescence microscopy with subtype-specific antibodies and by Western blot. Both dexmedetomidine, an alpha(2)-selective agonist, and norepinephrine, increased actin cytoskeleton organization and this increase was blocked by the alpha(2)-selective antagonist, atipamezole. Furthermore, dexmedetomidine inhibited isoproterenol-stimulated cAMP accumulation in isolated fetal rat heart and this was blocked by rauwolscine. Therefore, functional alpha(2)A and alpha(2)B subtypes are present in the fetal rat heart where they may have a role in cardiac development.

Full Text

Duke Authors

Cited Authors

  • Porter, AC; Svensson, SPS; Stamer, WD; Bahl, JJ; Richman, JG; Regan, JW

Published Date

  • February 14, 2003

Published In

Volume / Issue

  • 72 / 13

Start / End Page

  • 1455 - 1466

PubMed ID

  • 12535714

Pubmed Central ID

  • 12535714

International Standard Serial Number (ISSN)

  • 0024-3205

Digital Object Identifier (DOI)

  • 10.1016/s0024-3205(02)02381-0

Language

  • eng

Conference Location

  • Netherlands