Forskolin stimulation of water and cation permeability in aquaporin 1 water channels.

Journal Article (Journal Article)

Aquaporin 1, a six-transmembrane domain protein, is a water channel present in many fluid-secreting and -absorbing cells. In Xenopus oocytes injected with aquaporin 1 complementary RNA, the application of forskolin or cyclic 8-bromo- adenosine 3',5'-monophosphate increased membrane permeability to water and triggered a cationic conductance. The cationic conductance was also induced by direct injection of protein kinase A (PKA) catalytic subunit, reduced by the kinase inhibitor H7, and blocked by HgCl2, an inhibitor of aquaporin 1. The cationic permeability of the aquaporin 1 channel is activated by a cyclic adenosine monophosphate-dependent mechanism that may involve direct or indirect phosphorylation by PKA.

Full Text

Duke Authors

Cited Authors

  • Yool, AJ; Stamer, WD; Regan, JW

Published Date

  • August 30, 1996

Published In

Volume / Issue

  • 273 / 5279

Start / End Page

  • 1216 - 1218

PubMed ID

  • 8703053

International Standard Serial Number (ISSN)

  • 0036-8075

Digital Object Identifier (DOI)

  • 10.1126/science.273.5279.1216


  • eng

Conference Location

  • United States