Forskolin stimulation of water and cation permeability in aquaporin 1 water channels.
Journal Article (Journal Article)
Aquaporin 1, a six-transmembrane domain protein, is a water channel present in many fluid-secreting and -absorbing cells. In Xenopus oocytes injected with aquaporin 1 complementary RNA, the application of forskolin or cyclic 8-bromo- adenosine 3',5'-monophosphate increased membrane permeability to water and triggered a cationic conductance. The cationic conductance was also induced by direct injection of protein kinase A (PKA) catalytic subunit, reduced by the kinase inhibitor H7, and blocked by HgCl2, an inhibitor of aquaporin 1. The cationic permeability of the aquaporin 1 channel is activated by a cyclic adenosine monophosphate-dependent mechanism that may involve direct or indirect phosphorylation by PKA.
Full Text
Duke Authors
Cited Authors
- Yool, AJ; Stamer, WD; Regan, JW
Published Date
- August 30, 1996
Published In
Volume / Issue
- 273 / 5279
Start / End Page
- 1216 - 1218
PubMed ID
- 8703053
International Standard Serial Number (ISSN)
- 0036-8075
Digital Object Identifier (DOI)
- 10.1126/science.273.5279.1216
Language
- eng
Conference Location
- United States