Skip to main content
Journal cover image

In vivo and in vitro debromination of decabromodiphenyl ether (BDE 209) by juvenile rainbow trout and common carp.

Publication ,  Journal Article
Stapleton, HM; Brazil, B; Holbrook, RD; Mitchelmore, CL; Benedict, R; Konstantinov, A; Potter, D
Published in: Environmental science & technology
August 2006

Decabromodiphenyl ether (BDE 209), the major congener in the high volume industrial flame retardant mixture "DecaBDE", has recently been shown to be metabolized by carp. To further explore this phenomenon, juvenile rainbow trout were exposed to BDE 209 via the diet for a five month period. Analysis of the whole body homogenate, liver, serum, and intestinal tissues revealed that BDE 209 accumulated in rainbow trout tissues and was most concentrated in the liver. In addition to BDE 209, several hepta-, octa-, and nonaBDE congeners also accumulated in rainbow trout tissues over the same period as a result of BDE 209 debromination. Based on the total body burden of the hepta- through decaBDE congeners, uptake of BDE 209 was estimated at 3.2%. Congener profiles were different among whole body homogenate, liver, and serum, with the whole body homogenates having a greater contribution of the debrominated biotransformation products. Extracts of the rainbow trout whole body homogenates were compared with extracts from a previous experiment with common carp. This comparison revealed that BDE 202 (2,2',3,3',5,5',6,6'-octabromodiphenyl ether) was a dominant debromination product in both studies. To determine whether the observed debromination was metabolically driven, liver microsomal fractions were prepared from both common carp and rainbow trout. Analysis of the microsomal fractions following incubation with BDE 209 revealed that rainbow trout biotransformed as much as 22% of the BDE 209 mass, primarily to octa- and nonaBDE congeners. In contrast, carp liver microsomes biotransformed up to 65% of the BDE 209 mass, primarily down to hexaBDE congeners. These microsomal incubations confirm a metabolic pathway for BDE 209 debromination.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Environmental science & technology

DOI

EISSN

1520-5851

ISSN

0013-936X

Publication Date

August 2006

Volume

40

Issue

15

Start / End Page

4653 / 4658

Related Subject Headings

  • Water Pollutants, Chemical
  • Tissue Distribution
  • Polybrominated Biphenyls
  • Phenyl Ethers
  • Oncorhynchus mykiss
  • Microsomes, Liver
  • In Vitro Techniques
  • Halogenated Diphenyl Ethers
  • Environmental Sciences
  • Carps
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Stapleton, H. M., Brazil, B., Holbrook, R. D., Mitchelmore, C. L., Benedict, R., Konstantinov, A., & Potter, D. (2006). In vivo and in vitro debromination of decabromodiphenyl ether (BDE 209) by juvenile rainbow trout and common carp. Environmental Science & Technology, 40(15), 4653–4658. https://doi.org/10.1021/es060573x
Stapleton, Heather M., Brian Brazil, R David Holbrook, Carys L. Mitchelmore, Rae Benedict, Alex Konstantinov, and Dave Potter. “In vivo and in vitro debromination of decabromodiphenyl ether (BDE 209) by juvenile rainbow trout and common carp.Environmental Science & Technology 40, no. 15 (August 2006): 4653–58. https://doi.org/10.1021/es060573x.
Stapleton HM, Brazil B, Holbrook RD, Mitchelmore CL, Benedict R, Konstantinov A, et al. In vivo and in vitro debromination of decabromodiphenyl ether (BDE 209) by juvenile rainbow trout and common carp. Environmental science & technology. 2006 Aug;40(15):4653–8.
Stapleton, Heather M., et al. “In vivo and in vitro debromination of decabromodiphenyl ether (BDE 209) by juvenile rainbow trout and common carp.Environmental Science & Technology, vol. 40, no. 15, Aug. 2006, pp. 4653–58. Epmc, doi:10.1021/es060573x.
Stapleton HM, Brazil B, Holbrook RD, Mitchelmore CL, Benedict R, Konstantinov A, Potter D. In vivo and in vitro debromination of decabromodiphenyl ether (BDE 209) by juvenile rainbow trout and common carp. Environmental science & technology. 2006 Aug;40(15):4653–4658.
Journal cover image

Published In

Environmental science & technology

DOI

EISSN

1520-5851

ISSN

0013-936X

Publication Date

August 2006

Volume

40

Issue

15

Start / End Page

4653 / 4658

Related Subject Headings

  • Water Pollutants, Chemical
  • Tissue Distribution
  • Polybrominated Biphenyls
  • Phenyl Ethers
  • Oncorhynchus mykiss
  • Microsomes, Liver
  • In Vitro Techniques
  • Halogenated Diphenyl Ethers
  • Environmental Sciences
  • Carps