Escherichia coli produces linoleic acid during late stationary phase.
Journal Article (Journal Article)
Escherichia coli produces linoleic acid in the late stationary phase. This was the case whether the cultures were grown aerobically or anaerobically on a supplemented glucose-salts medium. The linoleic acid was detected by thin-layer chromatography and was measured as the methyl ester by gas chromatography. The linoleic acid methyl ester was identified by its mass spectrum. Lipids extracted from late-stationary-phase cells generated thiobarbituric acid-reactive carbonyl products when incubated with a free radical initiator. In contrast, extracts from log-phase or early-stationary-phase cells failed to do so, in accordance with the presence of polyunsaturated fatty acid only in the stationary-phase cells.
Full Text
Duke Authors
Cited Authors
- Rabinowitch, HD; Sklan, D; Chace, DH; Stevens, RD; Fridovich, I
Published Date
- September 1993
Published In
Volume / Issue
- 175 / 17
Start / End Page
- 5324 - 5328
PubMed ID
- 8366020
Pubmed Central ID
- PMC206585
International Standard Serial Number (ISSN)
- 0021-9193
Digital Object Identifier (DOI)
- 10.1128/jb.175.17.5324-5328.1993
Language
- eng
Conference Location
- United States