E-4-hydroxy-2-nonenal is cytotoxic and cross-links cytoskeletal proteins in P19 neuroglial cultures.
Journal Article (Journal Article)
Lipid peroxidation increases with age in brain and is elevated further in Alzheimer's disease. E-4-hydroxy-2-nonenal and malondialdehyde are products of lipid peroxidation that can adduct and cross-link protein. Neurofibrillary tangles, a feature of Alzheimer's disease composed mostly of tau protein, contain cross-linked and ubiquitin-conjugated protein. In P19 neuroglial cultures, E-4-hydroxy-2-nonenal was a potent cytotoxin that cross-linked cytoskeletal proteins, including tau into high molecular weight species that were conjugated with ubiquitin. Malondialdehyde formed monoadducts with cell protein but did not cross-link and was not cytotoxic. A non-crosslinking analogue of E-4-hydroxy-2-nonenal was not cytotoxic. E-4-Hydroxy-2-nonenal may contribute to neurodegeneration and neurofibrillary tangle formation in Alzheimer's disease.
Full Text
Duke Authors
Cited Authors
- Montine, TJ; Amarnath, V; Martin, ME; Strittmatter, WJ; Graham, DG
Published Date
- January 1996
Published In
Volume / Issue
- 148 / 1
Start / End Page
- 89 - 93
PubMed ID
- 8546230
Pubmed Central ID
- PMC1861611
International Standard Serial Number (ISSN)
- 0002-9440
Language
- eng
Conference Location
- United States