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E-4-hydroxy-2-nonenal is cytotoxic and cross-links cytoskeletal proteins in P19 neuroglial cultures.

Publication ,  Journal Article
Montine, TJ; Amarnath, V; Martin, ME; Strittmatter, WJ; Graham, DG
Published in: Am J Pathol
January 1996

Lipid peroxidation increases with age in brain and is elevated further in Alzheimer's disease. E-4-hydroxy-2-nonenal and malondialdehyde are products of lipid peroxidation that can adduct and cross-link protein. Neurofibrillary tangles, a feature of Alzheimer's disease composed mostly of tau protein, contain cross-linked and ubiquitin-conjugated protein. In P19 neuroglial cultures, E-4-hydroxy-2-nonenal was a potent cytotoxin that cross-linked cytoskeletal proteins, including tau into high molecular weight species that were conjugated with ubiquitin. Malondialdehyde formed monoadducts with cell protein but did not cross-link and was not cytotoxic. A non-crosslinking analogue of E-4-hydroxy-2-nonenal was not cytotoxic. E-4-Hydroxy-2-nonenal may contribute to neurodegeneration and neurofibrillary tangle formation in Alzheimer's disease.

Duke Scholars

Published In

Am J Pathol

ISSN

0002-9440

Publication Date

January 1996

Volume

148

Issue

1

Start / End Page

89 / 93

Location

United States

Related Subject Headings

  • tau Proteins
  • Tumor Cells, Cultured
  • Pathology
  • Neuroglia
  • Lipid Peroxidation
  • Cytoskeletal Proteins
  • Carcinoma, Embryonal
  • Aldehydes
  • 42 Health sciences
  • 32 Biomedical and clinical sciences
 

Citation

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Montine, T. J., Amarnath, V., Martin, M. E., Strittmatter, W. J., & Graham, D. G. (1996). E-4-hydroxy-2-nonenal is cytotoxic and cross-links cytoskeletal proteins in P19 neuroglial cultures. Am J Pathol, 148(1), 89–93.
Montine, T. J., V. Amarnath, M. E. Martin, W. J. Strittmatter, and D. G. Graham. “E-4-hydroxy-2-nonenal is cytotoxic and cross-links cytoskeletal proteins in P19 neuroglial cultures.Am J Pathol 148, no. 1 (January 1996): 89–93.
Montine TJ, Amarnath V, Martin ME, Strittmatter WJ, Graham DG. E-4-hydroxy-2-nonenal is cytotoxic and cross-links cytoskeletal proteins in P19 neuroglial cultures. Am J Pathol. 1996 Jan;148(1):89–93.
Montine, T. J., et al. “E-4-hydroxy-2-nonenal is cytotoxic and cross-links cytoskeletal proteins in P19 neuroglial cultures.Am J Pathol, vol. 148, no. 1, Jan. 1996, pp. 89–93.
Montine TJ, Amarnath V, Martin ME, Strittmatter WJ, Graham DG. E-4-hydroxy-2-nonenal is cytotoxic and cross-links cytoskeletal proteins in P19 neuroglial cultures. Am J Pathol. 1996 Jan;148(1):89–93.
Journal cover image

Published In

Am J Pathol

ISSN

0002-9440

Publication Date

January 1996

Volume

148

Issue

1

Start / End Page

89 / 93

Location

United States

Related Subject Headings

  • tau Proteins
  • Tumor Cells, Cultured
  • Pathology
  • Neuroglia
  • Lipid Peroxidation
  • Cytoskeletal Proteins
  • Carcinoma, Embryonal
  • Aldehydes
  • 42 Health sciences
  • 32 Biomedical and clinical sciences