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Reduced levels of human apoE4 protein in an animal model of cognitive impairment.

Publication ,  Journal Article
Sullivan, PM; Han, B; Liu, F; Mace, BE; Ervin, JF; Wu, S; Koger, D; Paul, S; Bales, KR
Published in: Neurobiol Aging
May 2011

The APOE4 allele is the most common genetic determinant for Alzheimer's disease (AD) in the developed world. APOE genotype specific differences in brain apolipoprotein E protein levels have been observed in numerous studies since the discovery of APOE4's link to AD. Since the human apoE4 targeted replacement mice display characteristics of cognitive impairment we sought to determine if reduced levels of apoE might provide one explanation for this impairment. We developed a novel mass spectrometry method to measure apoE protein levels in plasma. Additionally, we developed an ELISA that replicates the mass spectrometry data and enables the rapid quantitation of apoE in plasma, brain and cerebrospinal fluid. We detected a significant decrease in plasma, brain and cerebrospinal fluid apoE levels in the apoE4 mice compared to apoE2 and E3 mice. We also measured a small (∼19%) decrease in brain apoE levels from aged, non-demented APOE4 carriers. Our findings suggest that a fraction of APOE4-linked AD may be due to insufficient levels of functional apoE required to maintain neuronal health.

Duke Scholars

Published In

Neurobiol Aging

DOI

EISSN

1558-1497

Publication Date

May 2011

Volume

32

Issue

5

Start / End Page

791 / 801

Location

United States

Related Subject Headings

  • Neurology & Neurosurgery
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Mass Spectrometry
  • Male
  • Humans
  • Female
  • Enzyme-Linked Immunosorbent Assay
  • Disease Models, Animal
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Sullivan, P. M., Han, B., Liu, F., Mace, B. E., Ervin, J. F., Wu, S., … Bales, K. R. (2011). Reduced levels of human apoE4 protein in an animal model of cognitive impairment. Neurobiol Aging, 32(5), 791–801. https://doi.org/10.1016/j.neurobiolaging.2009.05.011
Sullivan, P. M., B. Han, F. Liu, B. E. Mace, J. F. Ervin, S. Wu, D. Koger, S. Paul, and K. R. Bales. “Reduced levels of human apoE4 protein in an animal model of cognitive impairment.Neurobiol Aging 32, no. 5 (May 2011): 791–801. https://doi.org/10.1016/j.neurobiolaging.2009.05.011.
Sullivan PM, Han B, Liu F, Mace BE, Ervin JF, Wu S, et al. Reduced levels of human apoE4 protein in an animal model of cognitive impairment. Neurobiol Aging. 2011 May;32(5):791–801.
Sullivan, P. M., et al. “Reduced levels of human apoE4 protein in an animal model of cognitive impairment.Neurobiol Aging, vol. 32, no. 5, May 2011, pp. 791–801. Pubmed, doi:10.1016/j.neurobiolaging.2009.05.011.
Sullivan PM, Han B, Liu F, Mace BE, Ervin JF, Wu S, Koger D, Paul S, Bales KR. Reduced levels of human apoE4 protein in an animal model of cognitive impairment. Neurobiol Aging. 2011 May;32(5):791–801.
Journal cover image

Published In

Neurobiol Aging

DOI

EISSN

1558-1497

Publication Date

May 2011

Volume

32

Issue

5

Start / End Page

791 / 801

Location

United States

Related Subject Headings

  • Neurology & Neurosurgery
  • Molecular Sequence Data
  • Mice, Transgenic
  • Mice
  • Mass Spectrometry
  • Male
  • Humans
  • Female
  • Enzyme-Linked Immunosorbent Assay
  • Disease Models, Animal