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Rapid and orthogonal logic gating with a gibberellin-induced dimerization system.

Publication ,  Journal Article
Miyamoto, T; DeRose, R; Suarez, A; Ueno, T; Chen, M; Sun, T-P; Wolfgang, MJ; Mukherjee, C; Meyers, DJ; Inoue, T
Published in: Nature chemical biology
March 2012

Using a newly synthesized gibberellin analog containing an acetoxymethyl group (GA(3)-AM) and its binding proteins, we developed an efficient chemically inducible dimerization (CID) system that is completely orthogonal to existing rapamycin-mediated protein dimerization. Combining the two systems should allow applications that have been difficult or impossible with only one CID system. By using both chemical inputs (rapamycin and GA(3)-AM), we designed and synthesized Boolean logic gates in living mammalian cells. These gates produced output signals such as fluorescence and membrane ruffling on a timescale of seconds, substantially faster than earlier intracellular logic gates. The use of two orthogonal dimerization systems in the same cell also allows for finer modulation of protein perturbations than is possible with a single dimerizer.

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Published In

Nature chemical biology

DOI

EISSN

1552-4469

ISSN

1552-4450

Publication Date

March 2012

Volume

8

Issue

5

Start / End Page

465 / 470

Related Subject Headings

  • Sirolimus
  • Protein Multimerization
  • NIH 3T3 Cells
  • Mice
  • Humans
  • Hela Cells
  • HeLa Cells
  • Gibberellins
  • Fluorescence
  • Biochemistry & Molecular Biology
 

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Miyamoto, T., DeRose, R., Suarez, A., Ueno, T., Chen, M., Sun, T.-P., … Inoue, T. (2012). Rapid and orthogonal logic gating with a gibberellin-induced dimerization system. Nature Chemical Biology, 8(5), 465–470. https://doi.org/10.1038/nchembio.922
Miyamoto, Takafumi, Robert DeRose, Allison Suarez, Tasuku Ueno, Melinda Chen, Tai-ping Sun, Michael J. Wolfgang, Chandrani Mukherjee, David J. Meyers, and Takanari Inoue. “Rapid and orthogonal logic gating with a gibberellin-induced dimerization system.Nature Chemical Biology 8, no. 5 (March 2012): 465–70. https://doi.org/10.1038/nchembio.922.
Miyamoto T, DeRose R, Suarez A, Ueno T, Chen M, Sun T-P, et al. Rapid and orthogonal logic gating with a gibberellin-induced dimerization system. Nature chemical biology. 2012 Mar;8(5):465–70.
Miyamoto, Takafumi, et al. “Rapid and orthogonal logic gating with a gibberellin-induced dimerization system.Nature Chemical Biology, vol. 8, no. 5, Mar. 2012, pp. 465–70. Epmc, doi:10.1038/nchembio.922.
Miyamoto T, DeRose R, Suarez A, Ueno T, Chen M, Sun T-P, Wolfgang MJ, Mukherjee C, Meyers DJ, Inoue T. Rapid and orthogonal logic gating with a gibberellin-induced dimerization system. Nature chemical biology. 2012 Mar;8(5):465–470.

Published In

Nature chemical biology

DOI

EISSN

1552-4469

ISSN

1552-4450

Publication Date

March 2012

Volume

8

Issue

5

Start / End Page

465 / 470

Related Subject Headings

  • Sirolimus
  • Protein Multimerization
  • NIH 3T3 Cells
  • Mice
  • Humans
  • Hela Cells
  • HeLa Cells
  • Gibberellins
  • Fluorescence
  • Biochemistry & Molecular Biology